The 786-O and A-498 cells (2 × 105) were seeded in 6-cm dishes and treated with melatonin, pazopanib, or both for 48 hours. Annexin V/propidium iodide (PI) staining and the mitochondrial membrane potential were detected using a Muse annexin V and dead cell assay kit and a Muse Mitopotential assay kit (Millipore), respectively. Then, the cells were analyzed using a Muse cell analyzer (Millipore).
Muse mitopotential assay kit
Manufactured by Merck Group
Sourced in United States, Germany, France
The Muse MitoPotential Assay Kit is a fluorescence-based tool used to measure the mitochondrial membrane potential (ΔΨm) in cells. It provides a quantitative assessment of this parameter, which is an indicator of mitochondrial health and function.
Automatically generated - may contain errors
Lab products found in correlation
Muse cell analyzer, by Merck Group (19 mentions)
Muse oxidative stress kit, by Merck Group (3 mentions)
Muse annexin 5 and dead cell assay kit, by Merck Group (2 mentions)
Muse cell analyzer assays, by Merck Group (1 mentions)
Mitotracker green fm, by Thermo Fisher Scientific (1 mentions)
Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions)
Cytoflex flow cytometer, by Beckman Coulter (1 mentions)
Muse multicaspase assay kit, by Merck Group (1 mentions)
Anti p21, by Abcam (1 mentions)
Anti sirt1, by Abcam (1 mentions)
Anti nrf2, by Abcam (1 mentions)
Anti ho 1, by Abcam (1 mentions)
Gkt137831, by Merck Group (1 mentions)
Anti bcl xl, by Merck Group (1 mentions)
Anti lamin b1, by Merck Group (1 mentions)
Ex 527, by Merck Group (1 mentions)
Anti rb, by Abcam (1 mentions)
Anti p rb, by Abcam (1 mentions)
Anti glut3, by Abcam (1 mentions)
Anti bax, by Merck Group (1 mentions)
29 protocols using muse mitopotential assay kit
1
Melatonin and Pazopanib Cytotoxicity
2
Mitochondrial Membrane Potential Assay
3
Mitochondrial Membrane Potential Measurement
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Oxidative stress was measured by Muse® oxidative stress kit (Millipore) in a Muse Cell Analyzer (Millipore, Bedford, MA, U.S.A.). Briefly, 1 × 106 cells were incubated with Muse® oxidative stress working solution at 37°C for 30 min and then mixed thoroughly before being analyzed on the cell analyzer. Changes in mitochondria membrane potential (ΔΨm) was measured using Muse™ MitoPotential assay kit (Millipore). This assay helped to differentiate four different populations of cells: live cells with the depolarized mitochondrial membrane, live cells with the intact mitochondrial membrane, MitoPotential+/7-AAD−; dead cells with the depolarized mitochondrial membrane, MitoPotential+/7-AAD+; and dead cells with the intact mitochondrial membrane, MitoPotential−/7-AAD+.
4
Mitochondrial Potential Assay of Physcion
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The Δψm reduction was analyzed with the Muse Mitopotential Assay kit (Merck Millipore). Cells treated with physcion (80–300 µM) were suspended in the Muse MitoPotential working solution and then incubated at 37 °C for 20 min. After incubation, cells were stained with 7-AAD at room temperature for 5 min. The stained cell suspension was analyzed by flow cytometry. The results were obtained from three independent replications.
5
Mitochondrial Membrane Potential Analysis
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Measurement of changes in mitochondrial membrane potential (ΔΨm) was performed with the MUSE™ MitoPotential Assay kit (Merck Millipore, Darmstadt, Germany). After treatment of the cells with astaxanthin, the culture medium was removed and EqASC cells were washed twice with HBSS, then incubated with the MitoPotential fluorescent dye for 30 min at 37 °C. The percentage of depolarized cells (depolarized alive + depolarized dead) was determined by Muse™ Cell Analyzer (Merck Millipore, Darmstadt, Germany).
6
Mitochondrial Potential Assay of Cancer Cells
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The analysis was conducted by using Muse Mitopotential Assay Kit (Merck Millipore). The SCC-9 and SCC-47 cells were collected at 1 × 105 per tube and treated for 24 h with different concentrations of platyphyllenone (0, 20, 30, 40 μM). After PBS washing, Muse MitoPotential working solution was added to the sample, which was reacted at 37 °C for 20 min. Muse MitoPotential 7-AAD was added soon afterwards, reacting for 5 min at room temperature, and Muse MitoPotential Analyzer and analysis data were obtained using the Muse® Cell Analyzer Assays (Merck Millipore). Each analysis was repeated in three separate experiments.
7
Mitochondrial Membrane Potential Analysis
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Changes in mitochondrial membrane potential were analyzed by Muse Cell Analyzer, using the Muse MitoPotential Assay Kit (Merck, Poland). PANC-1 cells were seeded at a density of 2 × 104 per well in 6-well plates. After 24 h of culturing in the standard medium, the cells were treated with 2.6 nm or 18 nm as indicated in Treatments in the concentrations range of 0.5–5 μg/mL or 5–100 μg/mL for 24 h. Then, PANC-1 cells were detached and incubated in 5% CO2 at 37°C with the MitoPotential working solution and Muse MitoPotential 7-AAD reagent according to the manufacturer's protocol (Merck, Poland) [30 ]. The results were analyzed using Muse 1.4 analysis software.
8
Yamogenin-Induced Mitochondrial Dysfunction
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The SKOV-3 cells were seeded in a 12-well plate (1 × 105 cells/well) and incubated with yamogenin at concentrations of 10.0–70.0 µg/mL. The concentration of ethanol added to the cells did not exceed 0.7% (v/v). After 24 h of the treatment, the cells were stained with Muse MitoPotential Assay Kit (Merck Millipore), and determination of the percentage of depolarized/live and dead cells was conducted with Muse Cell Analyzer. All the experiments were independently repeated three times.
9
Mitochondrial Potential Assay of Kigelia Extracts
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The SKOV-3 and HeLa cells (1 × 105 cells/well) were seeded in 12-well plates and incubated for 24 h. After this time, the water fraction of K. blossfeldiana was added to the cells at concentrations of 30, 60, and 100 µg/mL. The control was the cell group with 0.5% DMSO (v/v). After 24 h, the cells were washed with PBS and stained with the kit reagents from a Muse MitoPotential Assay Kit (Merck Millipore, Burlington, MA, USA), according to the manufacturer’s instruction [53 ]. A Muse Cell Analyzer (Merck Millipore, Burlington, MA, USA) was used to determine the percentage of live, depolarized live, depolarized dead, and dead cells. All experiments were independently repeated at least two times (in three repetitions).
10
Mitochondrial Membrane Potential Assay
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Changes in the mitochondrial membrane potential (MMP) were detected on the basis of a MitoPotential lipophilic cationic dye, combined to the 7-AAD dye as an indicator of cell death, using the Muse™ MitoPotential Assay kit (Merck Millipore, Darmstadt, Germany). After exposure to different concentrations of calystegines, i.e., 250 and 500 μg/ml or medium for control groups, EMS and healthy cells were washed with HBSS and stained with the fluorescent dyes for 30 min at 37 °C, and the percentage of depolarized cells (depolarized live + depolarized dead) was assessed by the mean of a Muse Cell Analyzer (Merck Millipore, Darmstadt, Germany).
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