Immunoblots were performed as described 74 (link). Samples were fractionated by SDS-PAGE and transferred to nitrocellulose membranes. The membrane was blocked in 50 mM Tris-buffered saline with 0.1% Tween-20 (TBST, pH 8.0) containing 4% nonfat milk for 1 hr at room temperature and probed with primary antibodies: 1) anti-HA-probe (Santa Cruz Biotechnology, Cat# sc-7392, 1:500), 2) anti-Ub (Santa Cruz Biotechnology, Cat# sc-8017) or anti-actin (Sigma-Aldrich, Cat# A2066, 1:1,000) in TBST containing 2% BSA at 4°C overnight. After washing 3X with TBST, the membranes were incubated with secondary antibodies: donkey IRDye 680RD anti-mouse (LI-COR Biosciences, Cat# 926–68072, 1:20,000), Goat IRDye 680RD anti-rabbit (LI-COR Biosciences, Cat# 926–68071, 1:20,000), and/or donkey IRDye800CW anti-mouse (LI-COR Biosciences, Cat# 926–32212, 1:20,000), goat IRDye 800CW α-rabbit (LI-COR Biosciences, Cat# 926–32211, 1:20,000) for 45 min at room temperature. Capture and analysis were performed using Li-Cor Odyssey CLX scanner and Image Studio software (LI-COR Biosciences).
Donkey irdye800cw anti mouse
Manufactured by LI COR
The Donkey IRDye800CW anti-mouse is a secondary antibody product designed for use in various immunoassay and imaging applications. It is conjugated with the IRDye800CW fluorescent dye, which enables detection and quantification of target proteins in a range of experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Odyssey clx scanner, by LI COR (2 mentions)
Anti actin, by Merck Group (2 mentions)
Anti ha probe, by Santa Cruz Biotechnology (2 mentions)
Irdye 680rd donkey anti mouse, by LI COR (2 mentions)
Irdye 680rd goat anti rabbit, by LI COR (2 mentions)
Goat α rabbit irdye 800cw, by LI COR (2 mentions)
Image studio software, by LI COR (2 mentions)
Anti ub, by Santa Cruz Biotechnology (1 mentions)
2 protocols using donkey irdye800cw anti mouse
1
Western Blot Quantification Protocol
2
Immunoblot Analysis of Ubiquitinated Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunoblots were performed as described 77 (link). Samples were fractionated by SDS-PAGE and transferred to nitrocellulose membranes. The membrane was blocked in 50 mM Tris-buffered saline with 0.1% Tween-20 (TBST, pH 8.0) containing 4% nonfat milk for 1 hr at room temperature and probed with primary antibodies: 1) anti-HA-probe (Santa Cruz Biotechnology, Cat# sc-7392, 1:500), 2) anti-Ub (FK1; Santa Cruz Biotechnology, Cat# sc-8017) or anti-actin (Sigma-Aldrich, Cat# A2066, 1:1,000) in TBST containing 2% BSA at 4°C overnight. After washing 3X with TBST, the membranes were incubated with secondary antibodies: donkey IRDye 680RD anti-mouse (LI-COR Biosciences, Cat# 926–68072, 1:20,000), Goat IRDye 680RD anti-rabbit (LI-COR Biosciences, Cat# 926–68071, 1:20,000), and/or donkey IRDye800CW anti-mouse (LI-COR Biosciences, Cat# 926–32212, 1:20,000), goat IRDye 800CW α-rabbit (LI-COR Biosciences, Cat# 926–32211, 1:20,000) for 45 min at room temperature. Capture and analysis were performed using Li-Cor Odyssey CLX scanner and Image Studio software (LI-COR Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!