We utilized the Cell Counting Kit-8 (CCK-8) assay to explore A375 cell proliferation. The transfected cells were planted at a density of 2000 cells/well in a 96-well plate overnight (at 37 °C with 5% CO2). The cells were incubated with 10 µL of CCK-8 labelling reagent (A311-01, vazyme, Nanjing, China) and 90 µL of serum-free medium per well in darkness at 37 °C for 2 h. Absorbance was determined at 450 nm wavelength with the enzyme-labelled meter (A33978, Thermo, Waltham, MA, USA). After that, A375 cells were stained with the EdU assay kit (Ribobio, Guangzhou, China) following the manufacturer’s directions. three randomly selected fields were photographed by a fluorescence microscope. Lastly, the EdU-positive cells were counted and quantified through the ImageJ software. Concerning the colony formation experiment, about 500 transfected A375 cells were seeded into each well of the 6-well plate and given time to form into colonies. Next, cells were washed with PBS and fixed with 4% paraformaldehyde for 15 min. Staining crystalline violet for 20 min, allowing it to dry at room temperature, and then counting the number of cells under an inverted microscope yielded the desired results.
A33978
Manufactured by Thermo Fisher Scientific
Sourced in United States
The A33978 is a laboratory centrifuge designed for general-purpose applications. It features a maximum speed of 6,000 RPM and can accommodate rotor configurations with a maximum capacity of 500 mL. The centrifuge is equipped with an automatic rotor identification system and a digital display for monitoring speed, time, and temperature. The A33978 is intended for use in a variety of laboratory settings to separate and isolate different components from liquid samples.
Automatically generated - may contain errors
Lab products found in correlation
A311 01, by Vazyme (9 mentions)
5 ethynyl 2 deoxyuridine edu assay, by RiboBio (2 mentions)
Crystal violet, by Solarbio (2 mentions)
Edu assay kit, by RiboBio (1 mentions)
Cck 8 labeling reagent, by Vazyme (1 mentions)
Cell counting kit 8 (cck8), by Vazyme (1 mentions)
5 ethynyl 2 deoxyuridine (edu), by Beyotime (1 mentions)
Cck 8 labelling reagent, by Dojindo Laboratories (1 mentions)
13 protocols using a33978
1
Evaluating A375 Cell Proliferation
2
Cell Viability Assay with CCK-8
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In 96-well plates, we seeded the cell suspension with 3×103 cells per well. The plate was then incubated for 2 hours at 37°C in the dark with 10 mL of CCK-8 labeling agent (A311-01, Vazyme) each well. The enzyme-labeled meter (A33978, Thermo) was used to measure the absorbance of the cells at 450 nm for 0, 24, 48, 72, and 96 hours in order to determine the vitality of the cells.
3
Cell Viability Assay of U87 and U251 Cells
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Cell viability of U87 MG cells and U251 MG cells after transfection was detected by CCK-8. The cell suspension was inoculated at a density of 5 × 103 cells per well in a 96-well plate and incubated for 24 h. CCK-8 marker (10 μL; A311-01, Vazyme) was added to each well, and incubated away from light at 37°C for 2 h. Cell viability was assessed by detecting the absorbance of the enzyme marker (A33978, Thermo) at 450 nm on days 1, 2, 3, and 4, respectively. The average OD values were calculated and plotted on a line graph.
4
Cell Viability Assessment using CCK-8
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Cells were plated in 96-well plates at a density of 1 × 103 cells per well, following standard protocols (45 (link), 46 (link)). Following that, the plates were incubated in darkness at 37°C for 2 hours with CCK-8 labeling reagent (A311-01, Vazyme). The assessment of cell viability was carried out by measuring the absorbance at 450 nm using an enzyme-linked spectrophotometer (A33978, Thermo) at time intervals of 0, 24, 48, 72, and 96 hours.
5
Cell Proliferation Assay with CCK-8
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The Cell Counting Kit-8 (CCK-8; Vazyme, Nanjing, China) was used to detect cell proliferation. We seeded the cells in 96-well plates at 2×103 cells per well. The plate was then incubated with 10 μl CCK-8 labeling reagent (A311-01, Vazyme, Nanjing, China) per well for 2 hours in the dark at 37°C. The absorbance of the cells was measured at 450 nm wavelength with the enzyme-labeled meter (A33978, Thermo, USA) to analyse the viability of the cells. It was detected for 0, 24, 48, 72, 96, and 120 hours.
6
Cell Viability Assay with CCK-8
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At first, 5×103 cells per well were seeded in the cell suspension in 96-well plates. All these cells were precultured. Following that, the plate was incubated with 10 mL of CCK-8 labeling solution (A311-01, Vazyme) in each well for 2 hours at 37° C in a dark environment. The enzyme-labeled meter (A33978, Thermo) was used to measure the cells’ absorbance at 450 nm in order to determine their health. Six measurements were made every 24 hours.
7
Cell Viability and Proliferation Assay
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The suspension of cells was seeded in 96-well plates at a density of 5×103 cells per well. After adding 10 μl of CCK-8 labeling agent (A311-01, Vazyme) to each well, the plate was incubated for 2 hours in the dark at 37°C. Cell viability was evaluated by measuring absorbance at 450 nm at 0, 24, 48, 72, and 96 hours using an enzyme-labeled meter (A33978, Thermo). The experiment was performed using a 96-well plate with 2×104 treated cells in each well, after the cells had adhered to the wall. The 5-Ethynyl-2’-deoxyuridine (EdU) assay was performed according to the manufacturer’s instructions (Ribobio, China), and cell proliferation was quantified using an inverted microscope.
8
Evaluating Cell Viability Post-Transfection
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Cell viability of 786-O and CAKI-1 cells post-transfection was assessed using the CCK-8 assay. Cell suspensions were seeded at a density of 5*103 cells per well in 96-well plates and cultured for 24 hours. Subsequently, 10 μL of CCK-8 reagent (A311-01, Vazyme) was added to each well and incubated in the dark at 37°C for 2 hours. Cell viability was determined by measuring absorbance at 450 nm using an enzyme marker (A33978, Thermo) on days 1, 2, 3, and 4. The mean OD values were computed and presented on a line graph.
9
Cell Proliferation Assay using CCK-8
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In this procedure, cells were plated in 96-well plates at a concentration of 3×10³ cells per well. Following cell seeding, 10 mL of CCK-8 solution (A311-01, Vazyme) was added to each well. The plates were then incubated in the dark at 37°C for 2 hours. The proliferation of cells was determined by measuring absorbance at 450 nm with a spectrophotometer (A33978, Thermo Fisher Scientific) at time points of 0, 24, 48, 72, and 96 hours.
10
Cell Proliferation Assay Protocol
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The cell suspension was seeded at a density of 3×103 cells per well in 96-well plates. After adding 10 μL of CCK-8 labeling agent (A311-01, Vazyme) to each well, the plate was incubated for 2 hours at 37°C in the dark. Absorbance at 450 nm was measured at 0, 24, 48, 72, and 96 hours using an enzyme-labeled meter (A33978, Thermo) to evaluate cell viability. Each well of a 6-well plate contained 1×103 transfected cells, and we maintained cell growth for 14 days. Two PBS washes and 15 minutes in 4% paraformaldehyde were performed before staining with Crystal Violet (Solarbio, China). Stained with 0.1% Crystal Violet. The experiment was performed after the cells had adhered to the wall using a 96-well plate with 2×104 treated cells in each well. The 5-Ethynyl-2’-deoxyuridine (EdU) assay was then performed according to the manufacturer’s instructions (Ribobio, China). Cell proliferation was quantified using an inverted microscope.
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