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Anti cd95 dx2

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Anti-CD95 [DX2] is a monoclonal antibody that binds to the CD95 (Fas) receptor on the surface of cells. It is used in research applications to study the role of the CD95 receptor in cellular processes.

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Lab products found in correlation

Anti cd3 sp34 2, by BD (3 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Anti ifn γ 4s b3, by BioLegend (2 mentions) Anti tnf α mab11, by BD (2 mentions) Anti cd28 cd28.2, by Beckman Coulter (2 mentions) Anti cd8 2st8.5h7, by Bio-Rad (2 mentions) Anti il 2 mq1 17h12, by BioLegend (2 mentions) Anti cd4 l200, by BD (1 mentions) Anti cd69 fn50, by BD (1 mentions) Anti cd20 2h7, by BD (1 mentions) Anti hla dr clone l243, by BD (1 mentions) Anti cd27 m t271, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

Anti-CD95 (10 citations) CD95 (DX2; (5 citations) APC anti-CD95 (clone DX2, (2 citations) Anti-CD95-PE-Cy5 (clone DX2, (2 citations)

3 protocols using anti cd95 dx2

1

Comprehensive Immunophenotyping of Blood Cells

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Immunophenotyping was performed on fresh blood at specified time points by flow cytometry using the following antibodies: anti-CD45 (clone D058–1283, Becton-Dickinson (BD) Le Pont-de-Claix, France), anti-CD3 (SP34–2, BD), anti-CD4 (L200, BD), anti-CD8 (BWl35/80, Miltenyi Biotec, Paris, France), anti-CD95 (DX2, BD), anti-CD28 (clone 28.2, Beckman Coulter), anti-CD69 (FN50, BD), anti-HLA-DR (clone L243, BD), anti-CD20 (2H7, BD), anti-CD27 (M-T271, Miltenyi), and anti-IgD (rabbit polyclonal, BioRad, Marnes-la-Coquette, France). Briefly, blood was incubated with the mixed antibodies for 15 min, red blood cells were lysed, and the cells were washed and fixed. A total of 105 cell events were acquired with a BD LSRII instrument and data was analysed using FlowJo software (Ashland, OR, USA).
Fovet C.M., Stimmer L., Contreras V., Horellou P., Hubert A., Seddiki N., Chapon C., Tricot S., Leroy C., Flament J., Massonneau J., Tchitchek N., 't Hart B.A., Zurawski S., Klucar P., Hantraye P., Deiva K., Zurawski G., Oh S., Le Grand R, & Serguera C. (2019). Intradermal vaccination prevents anti-MOG autoimmune encephalomyelitis in macaques. EBioMedicine, 47, 492-505.
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2

Multiparametric Flow Cytometry Immunophenotyping

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Surface stain monoclonal antibodies (mAbs) include: anti-CD4 [L200], anti-CD49d α4 integrin [9F10] and anti-CD95 [DX2] (BD Biosciences, San Jose, CA); anti-CD14 [TUK4], anti-CD20 [HI47] and LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen, Grand Island, NY); anti-CD28 [CD28.2] (Beckman Coulter, Pasadena, CA); anti-CD8 [2ST8.5H7] (Custom, mAb from Serotec, conjugation kit from Invitrogen).Intracellular stain mAbs include: anti-CD3 [SP34-2] and anti-TNF-α [MAb11] (BD Biosciences); anti-IFN-γ [4S.B3] and anti-IL-2 [MQ1-17H12] (Biolegend, San Diego CA).
Kutzler M.A., Wise M.C., Hutnick N.A., Moldoveanu Z., Hunter M., Reuter M., Yuan S., Yan J., Ginsberg A., Sylvester A., Pahar B., Carnathan D., Kathuria N., Khan A.S., Montefiori D., Sardesai N.Y., Betts M.R., Mestecky J., Marx P, & Weiner D.B. (2015). Chemokine Adjuvanted Electroporated-DNA Vaccine Induces Substantial Protection from Simian Immunodeficiency Virus Vaginal Challenge. Mucosal immunology, 9(1), 13-23.
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3

Multiparametric Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Surface stain monoclonal antibodies (mAbs) include: anti-CD4 [L200], anti-CD49d α4 integrin [9F10] and anti-CD95 [DX2] (BD Biosciences, San Jose, CA); anti-CD14 [TUK4], anti-CD20 [HI47] and LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen, Grand Island, NY); anti-CD28 [CD28.2] (Beckman Coulter, Pasadena, CA); anti-CD8 [2ST8.5H7] (Custom, mAb from Serotec, conjugation kit from Invitrogen).Intracellular stain mAbs include: anti-CD3 [SP34-2] and anti-TNF-α [MAb11] (BD Biosciences); anti-IFN-γ [4S.B3] and anti-IL-2 [MQ1-17H12] (Biolegend, San Diego CA).
Kutzler M.A., Wise M.C., Hutnick N.A., Moldoveanu Z., Hunter M., Reuter M., Yuan S., Yan J., Ginsberg A., Sylvester A., Pahar B., Carnathan D., Kathuria N., Khan A.S., Montefiori D., Sardesai N.Y., Betts M.R., Mestecky J., Marx P, & Weiner D.B. (2015). Chemokine Adjuvanted Electroporated-DNA Vaccine Induces Substantial Protection from Simian Immunodeficiency Virus Vaginal Challenge. Mucosal immunology, 9(1), 13-23.
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