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D2r rb af960

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The D2R-Rb-Af960 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is a specialized device designed for a specific function within the scientific research and analysis workflow. Due to the technical nature of the product, a detailed and unbiased description cannot be provided without the risk of making interpretations or extrapolations. As a marketing specialist, I do not have access to the comprehensive product information required to deliver a concise, factual, and unbiased description. Therefore, the description for this product is not available.

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Lab products found in correlation

Fluorophore conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Anti mcherry, by Thermo Fisher Scientific (2 mentions)

2 protocols using d2r rb af960

1

Immunostaining of Axonal Projections

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primary antibodies used were: anti-GFP (1:1000, rabbit, Abcam, catalog# ab13970, validated for both species and application by producer), anti-D2 (1:1000, mouse, Frontier Institute, catalog# D2R-Rb-Af960, validated for both species and application by producer), anti-mCherry (ThermoFisher Scientific, catalog# PA5–34974). Fluorophore-conjugated secondary antibodies were purchased from ThermoFisher Scientific. Antibodies were diluted in PBS with 10% NGS and PBST. No publications were found with rigorous validation of the anti-mCherry antibody for the application used in our study. However, the distribution of immunostained axonal projections resembled that of the endogenous mCherry fluorescence.
Gao C., Leng Y., Ma J., Rooke V., Rodriguez-Gonzalez S., Ramakrishnan C., Deisseroth K, & Penzo M.A. (2020). Two genetically, anatomically and functionally-distinct cell types segregate across the antero-posterior axis of the paraventricular thalamus. Nature neuroscience, 23(2), 217-228.
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2

Immunostaining of Axonal Projections

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primary antibodies used were: anti-GFP (1:1000, rabbit, Abcam, catalog# ab13970, validated for both species and application by producer), anti-D2 (1:1000, mouse, Frontier Institute, catalog# D2R-Rb-Af960, validated for both species and application by producer), anti-mCherry (ThermoFisher Scientific, catalog# PA5–34974). Fluorophore-conjugated secondary antibodies were purchased from ThermoFisher Scientific. Antibodies were diluted in PBS with 10% NGS and PBST. No publications were found with rigorous validation of the anti-mCherry antibody for the application used in our study. However, the distribution of immunostained axonal projections resembled that of the endogenous mCherry fluorescence.
Gao C., Leng Y., Ma J., Rooke V., Rodriguez-Gonzalez S., Ramakrishnan C., Deisseroth K, & Penzo M.A. (2020). Two genetically, anatomically and functionally-distinct cell types segregate across the antero-posterior axis of the paraventricular thalamus. Nature neuroscience, 23(2), 217-228.
+ Open protocol
+ Expand

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