Human bdnf elisa kit

Blood lipid particle sizes were measured using NMR spectroscopy,20 (link) a technique that simultaneously quantifies the number and sizes of very low-density lipoprotein (VLDL_NMR), low-density lipoprotein (LDL_NMR), and high-density lipoprotein (HDL_NMR) particles, expressing each as a lipoprotein particle concentration (particles/L), or as an average particle size measured in nanometers, [lipoScience, Chicago Illinois].21 (link) Serum BDNF levels were quantitatively determined using the human BDNF ELISA kit (Abcam, Cambridge, MA USA) as reported previously.13 (link)

The hADSCs were prepared as per detailed description in our previous study^{15 (link)
–17 (link, link)}
. The adipose tissues were digested with collagenase I, filtered through a
100-μm nylon sieve, and centrifuged at 470 g for 5 min. The pellet was
resuspended in Dulbecco’s modified Eagle’s medium (Invitrogen, Grand Island, NY,
USA) containing 0.2 mM ascorbic acid and 10% fetal bovine serum (FBS). The cell
suspension was recentrifuged at 470 g for 5 min, and the cell pellet was
collected. After overnight culture, nonadherent cells were removed by washing
with phosphate-buffered saline (PBS). The cell medium was changed to
keratinocyte-serum-free medium (SFM; Invitrogen) containing 0.2 mM ascorbic
acid, 0.09 mM calcium, 5 ng/mL recombinant EGF, and 5% FBS. The cells were
maintained for 4–5 days until confluent (passage 0). When the cells reached 90%
confluence, they were subculture expanded in keratinocyte-SFM medium until
passage 3. Karyotype analysis of the hADSCs was processed from GenDix (Seoul,
Korea). The BDNF and NGF levels in the conditioned medium produced by 1 ×
10⁶ hADSCs were determined using enzyme-linked immunosorbent
assay (ELISA). Human BDNF ELISA kit (ab99978, Abcam, Cambridge, UK) and human
NGF ELISA kit (ab99986, Abcam) were used according to the manufacturer’s
instructions.