Cells or tissues were lysed in RIPA buffer, and the protein concentration was determined by BCA protein assay. Nucleus extraction from total lysis was performed using Subcellular Structure Cytoplasmic and Nucleus Extraction Kit. Then proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). After electrophoresis, the proteins were electrotransferred to PVDF membrane (Millipore, Billerica, USA) and then blocked with 5% nonfat milk for 4 h. The primary antibodies are against DSC2 (#53485, Santa Cruz Biotechnology), MMP9 (#AF5228, Affbiotech), CD44 (#15675, Proteintech), N-cadherin (#22018, Proteintech), BRD4 (#DF2905, Affbiotech), Snail (#AF6032, Affbiotech), γ-catenin (#66445, Proteintech), β-catenin (#G0709, Santa Cruz Biotechnology), Lamin B1 (internal reference of nucleus, #AF5161, Affbiotech), and β-actin (internal reference of total cell, #ZF-0313, ZS Bio).
γ catenin
Manufactured by Proteintech
Sourced in United States, China
γ-catenin is a protein that plays a role in cell-cell adhesion and signal transduction. It is a component of the cadherin-catenin complex, which is important for the structural and functional integrity of cell-cell junctions.
Automatically generated - may contain errors
Lab products found in correlation
Pvdf membrane, by Merck Group (2 mentions)
β actin, by ZSGB-BIO (2 mentions)
N cadherin, by Proteintech (1 mentions)
β catenin, by Santa Cruz Biotechnology (1 mentions)
Cyto c, by Cell Signaling Technology (1 mentions)
P pi3k, by ABclonal (1 mentions)
Caspase 3, by Proteintech (1 mentions)
P akt, by Proteintech (1 mentions)
Bcl 2, by Proteintech (1 mentions)
2 protocols using γ catenin
1
Protein Expression Analysis of Cell Lines
2
Western Blot Analysis of Signaling Proteins
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Cells or tissues were lysed in RIPA buffer and protein concentration was determined by BCA protein assay. Nuclear extraction from total lysis was performed using Subcellular Structure Cytoplasmic and Nuclear Extraction Kit. Then, proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). After electrophoresis, the proteins were electrotransferred to PVDF membrane (Millipore, Billerica, USA) and then blocked with 5% non-fat milk for 4 h. The primary antibodies against DSC2 (#53485, Santa Cruz Biotechnology, USA), p-PI3K (#0854, ABclonal Technology, Wuhan, China), PI3K (#4255, Cell Signaling Technology, Massachusetts, USA), p-AKT (#66444, Proteintech, Wuhan, China), Akt (#60203, Proteintech, Wuhan, China), Cyto-C (#4280, Cell Signaling Technology, Massachusetts, USA), caspase-3 (#66470, Proteintech, Wuhan, China), BCL-2 (#12789, Proteintech, Wuhan, China), p53 (#AF0879, Affinity Biotechnology, USA), PTEN (#60300, Proteintech, Wuhan, China), γ-catenin (#66445, Proteintech, Wuhan, China), Lamin B1 (internal reference for the nucleus, #AF5161, Affinity Biotechnology, USA), and β-actin (internal reference for the total cell, #ZF-0313, ZS Bio, Beijing, China) were used.
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