Cba human anaphylatoxin kit

Manufactured by BD

Sourced in United States

The BD CBA Human Anaphylatoxin Kit is a laboratory test designed to measure the levels of anaphylatoxins, which are biomarkers associated with inflammatory processes, in human samples. The kit utilizes flow cytometry technology to provide quantitative results.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using cba human anaphylatoxin kit

Mechanism of GL-2045 Complement Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 2

Experiments were performed to determine the mechanisms by which GL-2045 protected cells from CDC. In a cell free system, normal human serum (NHS) was incubated with increasing concentrations of GL-2045, HAGG, and IVIG (1-10,000 μg/mL) for 90 minutes at 37° C. Levels of complement split products C4a, C3a, and C5a were evaluated with the BD Biosciences CBA human anaphylatoxin kit (cat #561418). In this system, GL-2045 mediated significant cleavage of C4, indicated by an increase in C4a (FIG. 2, left panel), and modest cleavage of C3, indicated by a smaller increase in C3a (FIG. 2, middle panel). Further, serum treated with GL-2045 did not contain detectable levels of C5a (FIG. 2, right panel). These data demonstrate that GL-2045 activates the initial steps of classical complement activation, as demonstrated by C4a production, has a limited ability to mediate downstream C3 cleavage, and is unable to mediate C5 cleavage at the doses tested. The results indicated that GL-2045 drove limited initial complement activation with an inability to mediate downstream activation.

US11331372B2. Methods of treating inflammatory disorders with multivalent Fc compounds (2022-05-17). Gliknik Inc. [US]. Inventors: David S. Block [US], Henrik Olsen [US].

+ Open protocol

+ Expand

Placental Inflammatory and Complement Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Interleukin (IL-) 1β, IL-6, IL-8, IL10, IL-12p70, and TNF-α were detected and quantified in placental plasma by CBA Human Inflammatory kit (BD Biosciences), according to the manufacturer’s protocol. Complement (C3a, C4a, and C5a) activation was evaluated in placental plasma through CBA Human Anaphylatoxin kit (BD Biosciences). Samples were analyzed in a two-laser BD FACSCalibur flow cytometer with CellQuest version 5.2 software (BD Biosciences) and concentrations computed using FCAP array software version 3.0.1 (BD Biosciences).

Dombrowski J.G., Barateiro A., Peixoto E.P., Barros A.B., de Souza R.M., Clark T.G., Campino S., Wrenger C., Wunderlich G., Palmisano G., Epiphanio S., Gonçalves L.A, & Marinho C.R. (2021). Adverse pregnancy outcomes are associated with Plasmodium vivax malaria in a prospective cohort of women from the Brazilian Amazon. PLoS Neglected Tropical Diseases, 15(4), e0009390.

+ Open protocol

+ Expand

Nanocarrier Complement Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nanocarrier formulations (10 mg mL⁻¹) were added to pooled complement human serum (Innovative Research) in a 1:20 ratio and incubated at 37 °C, 200 rpm for 1 h. Afterward, samples were treated with 5 × 10⁻³ m EDTA to halt complement activation and were placed on ice. The C3a, C4a, and C5a human anaphylatoxin concentrations were quantitatively assessed using a BD™ CBA Human Anaphylatoxin Kit. The C3a, C4a, and C5a standards and were prepared following the manufacturer’s protocol. Data acquisition was completed using a BD LSRFortessa. Analysis proceeded using Cytobank software.

Vincent M.P., Karabin N.B., Allen S.D., Bobbala S., Frey M.A., Yi S., Yang Y, & Scott E.A. (2021). The Combination of Morphology and Surface Chemistry Defines the Immunological Identity of Nanocarriers in Human Blood. Advanced therapeutics, 4(4), 2100062.

+ Open protocol

+ Expand

Quantifying Complement Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

1.5-ml polypropylene tubes were blocked with 30% heat-inactivated FBS at 4 °C o/n. Crystals (1 mg) were incubated in 50 µl of serum in these blocked tubes for 30 min at 37 °C with mild agitation. The supernatant was harvested and C3a, C4a, and C5a concentration was determined using the BD CBA human anaphylatoxin kit (BD Biosciences, 561418, sample dilution 1:15,000) and sC5b-9 concentration was determined using human TCC ELISA (Hycultec GmbH, HK328-02, sample dilution 1:750) according to the manufacturer’s recommendations.

Wessig A.K., Hoffmeister L., Klingberg A., Alberts A., Pich A., Brand K., Witte T, & Neumann K. (2022). Natural antibodies and CRP drive anaphylatoxin production by urate crystals. Scientific Reports, 12, 4483.

+ Open protocol

+ Expand

Non-invasive Tear Fluid Collection and Analysis

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The tear fluid was collected non-invasively during the ocular surface evaluation as reported previously^{27 (link)}. The same investigator (HM) collected the tear fluid in all cases. To avoid the effect of eye drops on the ocular surface measurements, participants were instructed not to use any eye drops on the day of tear fluid sample collection. The patient lay on his/her back on the bed and the clinician used a micropipette (Drummond Scientific, Broomall, PA, USA) to collect 2 μl of tear fluid from the external eyelid margins. The collected tear fluid was immediately stored at − 80 °C until analysis. The collected tears were measured using the BD CBA Human Anaphylatoxin Kit (BD Biosciences, San Jose, CA, USA) for CAPs (C3a, C4a, and C5a). The CAPs in the tear fluid were measured according to the previous reports and the manufacturer's instructions^{17 (link),22 (link)}.

Maehara H., Norikawa K., Tanaka K., Kato Y., Kasai A., Mukai R., Omori T., Machida T., Sekine H, & Sekiryu T. (2024). Complement activation products in tears of dry eye and meibomian gland dysfunction. Scientific Reports, 14, 43.

+ Open protocol

+ Expand

Measuring Inflammatory Biomarkers in COVID-19

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cytokines (IL-12, IL-1β, IL-6, IL-8, and TNF-α), chemokines (CCL5, CCL2, CXCL9, and CXCL10), and anaphylatoxins (C3a, C4a, and C5a) were measured with BD cytometric bead array (CBA) Human Inflammatory Cytokines Kit (Catalog No. 551811, BD), BD CBA Human Chemokine Kit (Catalog No. 552990, BD), and BD CBA Human Anaphylatoxin Kit (Catalog No. 561418, BD), respectively. All kits were acquired with LSR-Fortessa X20 (BD) and analyzed with FCAP Array Software v3.0 (BD Biosciences). Antibodies were measured with MAGLUMI 2019-nCoV IgM Kit (SNIBE) and MAGLUMI 2019-nCoV IgG Kit (SNIBE) (≥1.00 AU/ml) in a MAGLUMI 800 (SNIBE).

Sanhueza S., Vidal M.A., Hernandez M.A., Henriquez-Beltran M.E., Cabrera C., Quiroga R., Antilef B.E., Aguilar K.P., Castillo D.A., Llerena F.J., Fraga Figueroa M., Nazal M., Castro E., Lagos P., Moreno A., Lastra J.J., Gajardo J., Garcés P., Riffo B., Buchert J., Sanhueza R., Ormazába V., Saldivia P., Vargas C., Nourdin G., Koch E., Zuñiga F.A., Lamperti L., Bustos P., Guzmán-Gutiérrez E., Tapia C.A., Ferrada L., Cerda G., Woehlbier U., Riquelme E., Yuseff M.I., Muñoz Ramirez B.A., Lombardi G., De Gonzalo-Calvo D., Salomon C., Verdugo R.A., Quiñones L.A., Colombo A., Barría M.I., Labarca G, & Nova-Lamperti E. (2023). Clinical and pulmonary function analysis in long-COVID revealed that long-term pulmonary dysfunction is associated with vascular inflammation pathways and metabolic syndrome. Frontiers in Medicine, 10, 1271863.

+ Open protocol

+ Expand

Quantification of Complement Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The presence of C3a/C3a-desArg, C4a/C4a-desArg and C5a/C5a-desArg in the samples of plasma collected, as described above, was evaluated using the BD CBA Human Anaphylatoxin Kit, following the manufacturer’s instructions (BD Biosciences, California, USA). The presence of the soluble complement terminal complex (sTCC, SC5b-9) was evaluated by ELISA in plasma samples from human whole blood assays using the MicroVue SC5b-9 Plus EIA kit following the manufacturer’s instructions (Quidel Corporation, California, USA).

Leonel T.B., Gabrili J.J., Squaiella-Baptistão C.C., Woodruff T.M., Lambris J.D, & Tambourgi D.V. (2022). Bothrops jararaca Snake Venom Inflammation Induced in Human Whole Blood: Role of the Complement System. Frontiers in Immunology, 13, 885223.

+ Open protocol

+ Expand

Aqueous Humor Anaphylatoxin and Cytokine Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Levels of anaphylatoxins (C3a, C4a, and C5a) in the aqueous humor were measured using a BD CBA Human Anaphylatoxin Kit (BD Biosciences, San Jose, CA, USA). The levels of cytokines (VEGF and MCP-1) in the aqueous humor were measured using a BD Human Soluble Protein Master Buffer Kit (BD Biosciences). The bead-based immunoassays were performed according to the manufacturer's instructions.

Kato Y., Oguchi Y., Omori T., Shintake H., Tomita R., Kasai A., Ogasawara M., Sugano Y., Itagaki K., Ojima A., Machida T., Sekine H, & Sekiryu T. (2020). Complement Activation Products and Cytokines in Pachychoroid Neovasculopathy and Neovascular Age-Related Macular Degeneration. Investigative Ophthalmology & Visual Science, 61(13), 39.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!