Anti ets1

Manufactured by Active Motif

Anti-Ets1 is a laboratory reagent that can be used to detect and quantify the Ets1 protein, a transcription factor involved in various cellular processes. It functions as a specific antibody that binds to the Ets1 protein, enabling its identification and measurement in biological samples.

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Lab products found in correlation

Anti oct4, by Abcam (1 mentions) Anti nanog, by Active Motif (1 mentions) Anti klf4, by Abcam (1 mentions) Anti sox2, by Abcam (1 mentions) Anti ctcf, by Merck Group (1 mentions) Anti brd4, by Fortis Life Sciences (1 mentions) Anti cebpb, by Abcam (1 mentions) Anti ikaros, by Active Motif (1 mentions) Anti p300, by Active Motif (1 mentions) Anti pax5, by Abcam (1 mentions) Histone h3 23 34 peptide, by AnaSpec (1 mentions) Kaarksapatgg, by AnaSpec (1 mentions) Histone h3k27ac 23 34 peptide, by AnaSpec (1 mentions) Kaar k ac sapatgg, by AnaSpec (1 mentions) H3k27ac, by Active Motif (1 mentions) Anti h3k27ac, by Abcam (1 mentions) Anti h3k27me3, by Abcam (1 mentions) Rabbit polyclonal igg, by BioLegend (1 mentions)

2 protocols using anti ets1

Histone Modifications and Chromatin Interactions

Cited 2 times

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Rabbit polyclonal to H1, HMGN1, HMGN2, and H3 were from our laboratory, anti H3K27ac (Abcam#ab4729), anti H3K27me3 (Abcam#ab6002), monoclonal anti H1(Milipore-Sigma #05-457), anti CEBPB (Abcam#ab32358), Anti-Brd3 (Active Motif #61489), Anti-Brd4 (Bethyl Laboratories #A301-985A100), Anti-CEBPB (Abcam #ab32358), Anti-CTCF (EMD Millipore #07-729), Anti-Ets1 (Active Motif #39580), Anti-Ikaros (Active Motif #39355), Anti-Irf8 (Bethyl Laboratories #A304-027A), Anti-Klf4 (Abcam #106629), Anti-Nanog (Active Motif #61419), Anti-Oct4 (Abcam #ab19857), Anti-p300 (Active Motif #61401), Anti-Pax5 (Abcam #183575), Anti-Sox2 (Abcam #97959).
The following recombinant mononucleosomes were purchased from Active Motif: unmodified (#81070); H3K27me3 modified (#81834), H3K27ac modified (#81077).
Wild type and HMGN DKO mouse embryonic fibroblasts, embryonic stem cell lines^{55 (link)} and resting B cells^{56 (link)} were as previously described. Peptides Histone H3 (23–34) peptide, KAARKSAPATGG and Histone H3K27ac (23–34) peptide, KAAR - K(Ac)—SAPATGG were from AnaSpec, Inc.

Zhang S., Postnikov Y., Lobanov A., Furusawa T., Deng T, & Bustin M. (2022). H3K27ac nucleosomes facilitate HMGN localization at regulatory sites to modulate chromatin binding of transcription factors. Communications Biology, 5, 159.

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Chromatin Immunoprecipitation and qPCR Analysis

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ChIP was performed as reported previously (63 (link)), using the fixation and sonication conditions optimized for each cell type (Supplemental Table 3). DNA was immunoprecipitated using 5 μg of the following antibodies: anti-ETS1 (catalog 39580; Active Motif; targets ETS family member SPDEF), anti-SPDEF (catalog MBS2518460; myBiosource), or rabbit polyclonal IgG (catalog 910801; BioLegend). Crosslink-reversed and purified ChIP DNA was analyzed by qPCR, with relative factor occupancy calculated as described (63 (link)). Sequences of primers used for ChIP-qPCR are shown in Supplemental Table 4.

Gally F., Sasse S.K., Kurche J.S., Gruca M.A., Cardwell J.H., Okamoto T., Chu H.W., Hou X., Poirion O.B., Buchanan J., Preissl S., Ren B., Colgan S.P., Dowell R.D., Yang I.V., Schwartz D.A, & Gerber A.N. (2021). The MUC5B-associated variant rs35705950 resides within an enhancer subject to lineage- and disease-dependent epigenetic remodeling. JCI Insight, 6(2), e144294.

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