Biphenyl 100 column

Reaction products were analyzed using an Agilent 1260 Infinity HPLC system with UV detector. Extracted steroid products were dissolved in 20 μL of methanol, and 5 μL injections were resolved with a 50 × 2.1 mm, 2.6 μm, biphenyl 100 Å column (Phenomenex, Torrance, CA), equipped with a guard column at a flow rate of 0.4 mL/min. A methanol/water linear gradient was used: 32.5% to 88.3% methanol from 0 to 10.6 min, 88.3% to 12 min, 99.1% from 12.1 min to 14 min, and 32.5% from 14.1 min to 15 min. Products were identified by retention times of external standards chromatographed at the beginning and ends of the experiments, and the data were processed with Laura4 software (LabLogic) and graphed with GraphPad Prism 7 (GraphPad Software, San Diego, CA).

Reaction products were analyzed using an Agilent 1260 Infinity HPLC system with UV detector. Extracted steroid products were dissolved in 20 μL of methanol, and 5 μL injections were resolved with a 50 × 2.1 mm, 2.6 μm, biphenyl 100 Å column (Phenomenex, Torrance, CA), equipped with a guard column at a flow rate of 0.4 mL/min. A methanol/water linear gradient was used: 32.5% to 88.3% methanol from 0 to 10.6 min, 88.3% to 12 min, 99.1% from 12.1 min to 14 min, and 32.5% from 14.1 min to 15 min. Products were identified by retention times of external standards chromatographed at the beginning and ends of the experiments, and the data were processed with Laura4 software (LabLogic) and graphed with GraphPad Prism 6 (GraphPad Software, San Diego, CA).