Osteo assay surface for bone resorption

RAW264.7 cells were obtained from the American Type Culture Collection (Rockville, MD, USA). Recombinant mouse RANKL and recombinant mouse M-CSF were purchased from R&D Systems (Minneapolis, MN, USA). The Osteo Assay Surface for Bone Resorption was purchased from Corning (NY, USA). The TRAP stain kit was obtained from Sigma-Aldrich (NY, USA). The Actin Cytoskeleton and Focal Adhesion Staining Kit was purchased from Millipore (Darmstadt, Germany). Alpha minimal essential medium (α-MEM) and fetal bovine serum (FBS) were purchased from Gibco (Life Technologies, USA). Penicillin-streptomycin solution was obtained from Hyclone (Thermo Scientific, USA). Membrane dye DiI and Cell Tracker Green were obtained from Life Technologies.

Recombinant Mouse RANKL and Recombinant Mouse M-CSF were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies against AIF, cytochrome c, cleaved caspase-3, Bax, Bcl-2 and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Osteo Assay Surface for Bone Resorption was purchased from Corning (Corning, NY, USA). Bovine cortical bone slices were obtained from Boineslices.com (Jelling, Denmark). Cell Counting Kit-8 was obtained from Dojindo Molecular Technologies (Dojindo, Japan). The TRAP stain kit was obtained from Sigma-Aldrich (St. Louis, MO, USA). The Actin Cytoskeleton and Focal Adhesion Staining Kit was purchased from Millipore (Darmstadt, Germany). The DCFDA cellular ROS detection assay kit was obtained from ABcam (Cambridge, UK). Alpha minimal essential Medium (α-MEM) and fetal bovine serum (FBS) were purchased from Gibco (Life Technologies, Carlsbad, CA, USA). Penicillin–streptomycin solution was obtained from Hyclone (Thermo Scientific, Waltham, MA, USA). DHA was purchased from Sigma-Aldrich.