D glucose

Manufactured by Helicon

D-glucose is a monosaccharide, a type of simple sugar. It is a core component of many carbohydrates and serves as a primary source of energy for many organisms.

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2 protocols using d glucose

Yeast Strain Generation by Homologous Recombination

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Deletion and GFP-fusion strains were obtained by homologous recombination of the PCR product with heterologous selection markers, as listed in Table 1. All strains in this study are derivatives of the W303 strain. As templates for PCR, the DNA of yeast strains from Euroscarf collection, pUG27 (Gueldener et al., 2002 (link)) and pFA6a-His3MX6 (Longtine et al., 1998 (link)) plasmids were used. All newly generated strains were verified by PCR with independently designed primers (Table 2) and RT-qPCR (Supplementary Text S1, Table S1, Figure S1). We used standard yeast-rich and synthetic mediums described by Sherman (2002) (link). We obtained yeast extract from BD and D-glucose from Helicon. Clotrimazole, miconazole, nigericin, amphotericin B, propanol, butanol, and FM4–64 were obtained from Thermo Fisher Scientific. Bacto Agar, peptone, NaCl, KCl, and NaN₃ were obtained from Amresco, and 2-Deoxy-D-glucose was obtained from Chem-Impex Int’l Inc.

Sokolov S.S., Vorobeva M.A., Smirnova A.I., Smirnova E.A., Trushina N.I., Galkina K.V., Severin F.F, & Knorre D.A. (2020). LAM Genes Contribute to Environmental Stress Tolerance but Sensibilize Yeast Cells to Azoles. Frontiers in Microbiology, 11, 38.

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Transient Expression of Biosensors in Mouse Hippocampal Neurons

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The mixed mouse primary embryonic hippocampal neuronal cell culture was obtained as described previously [52 (link)]. These cells were cultured in Minimal Essential Medium (MEM, Sigma), containing penicillin/streptomycin (1%, Gibco), Fetal Bovine Serum (FBS, 5%, BioloT), HEPES (10 mM, PanEko), d-Glucose (0,6%, Helicon), Sodium Bicarbonate (2 g/L, Dia-M), GlutaMax (1%, Gibco) and B27 supplement (2%, Gibco) at 37 °C in 5% CO₂ and seeded on 35 mm glass coverslips, which were treated with 0,1 mg/ml Poly-d-Lysine. For transient expression of biosensors, we used AAV-based vectors with cloned HyPer7, SypHer3s or SoNar genes. Cells were infected with viral particles at an MOI of 10 000 VG/cell.

Kelmanson I.V., Shokhina A.G., Kotova D.A., Pochechuev M.S., Ivanova A.D., Kostyuk A.I., Panova A.S., Borodinova A.A., Solotenkov M.A., Stepanov E.A., Raevskii R.I., Moshchenko A.A., Pak V.V., Ermakova Y.G., van Belle G.J., Tarabykin V., Balaban P.M., Fedotov I.V., Fedotov A.B., Conrad M., Bogeski I., Katschinski D.M., Doeppner T.R., Bähr M., Zheltikov A.M., Bilan D.S, & Belousov V.V. (2021). In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model. Redox Biology, 48, 102178.

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