Hplc grade acetonitrile and methanol

Alternariol (AOH) was obtained from Cfm Oskar Tropitzsch (Marktredwitz, Germany). Racemic warfarin (WAR), naproxen (NAP), human serum albumin (HSA), bovine serum albumin (BSA), porcine serum albumin (PSA), rat serum albumin (RSA), glimepiride (GLIM), furosemide (FUR), bilirubin (BIL), phenylbutazone (PBut), indomethacin (IME), racemic ibuprofen (IBU) and S-camptothecin (CPT) were purchased from Sigma-Aldrich (Budapest, Hungary). Ethinylestradiol (EE) was purchased from Serva (Budapest, Hungary). Spectroscopic grade ethanol (96%), as well as HPLC-grade acetonitrile and methanol were obtained from VWR (Budapest, Hungary). Stock solutions of AOH (5000 μM), bilirubin (500 μM), methyl orange (2000 μM), and glimepiride (2000 μM) were prepared in spectroscopic grade dimethyl sulfoxide (DMSO; Fluka, Bucharest, Romania). Stock solutions of indomethacin and ethinylestradiol (both 2000 μM), as well as ibuprofen, furosemide, phenylbutazone, and naproxen (each 2500 μM) were prepared in ethanol (96%, spectroscopic grade). The applied amounts of organic solvents did not affect significantly the fluorescence measurements (tested in each spectroscopic model). All stock solutions were stored at −20 °C, and protected from light.

A local Irish farmer (Co. Kildare, Ireland) provided defatted hempseed cake following a cold oil extraction process. The hempseed cake pellets were ground (Lloytron E5012WI, Kitchen Perfected Blender, Leigh, UK), vacuum-packed, and preserved under refrigeration conditions (4 °C) until the application of further technological processing or chemical analyses.
All the chemicals used in this study were of analytical grade. The reagents used during the technological processing of the biomass include sodium hydroxide (NaOH) purchased from Sigma-Aldrich (St. Louis, MO, USA), hydrochloric acid (HCl, 37 % w/v) purchased from Honeywell-Fluka (Austria), and ultrapure water (Milli-Q System, Millipore, Burlington, MA, USA). For amino acid analysis, an amino acid calibration standard mix was purchased from Sigma Aldrich (Copenhagen, Denmark), and HPLC grade acetonitrile and methanol were obtained from VWR International (Søborg, Denmark). Other materials and reagents for SDS-PAGE analyses include NuPAGE^® LDS sample buffer, NuPAGE^® sample reducing agent, NuPAGE^® 4–12% Bis-Tris Gel 1.0 mm, NuPAGE^® antioxidant (Invitrogen, Life Technologies Corp., Waltham, CA, USA), PageRuler pre-stained protein ladder (Thermo Scientific, Vilnius, Lithuania), and Ready BlueTM (Sigma-Aldrich, Darmstadt, Germany).