Cd8 coated magnetic microbeads

Mononuclear cells were isolated from peripheral blood samples of healthy adult donors (n = 3) using Ficoll‐Paque PLUS (GE Healthcare Bio‐Sciences, Uppsala, Sweden) gradient centrifugation as previously described (Vereide et al., 2014). Peripheral blood mononuclear cells at a density of 1 × 10⁷ per mL were incubated with CD8‐coated magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for 15 min at 4 °C. The cell suspension was loaded onto a magnetic activated cell sorter (MACS) column placed in the magnetic field of a MACS separator. The magnetically labeled CD8⁺ T cells were retained within the column during washing with PBS. The CD8⁺ T cells were then eluted after removing the column from the magnetic field.

Peripheral blood mononuclear cells were isolated from healthy adult donors using Ficoll‐Paque™ PLUS (GE Healthcare Bio‐Sciences, Uppsala, Sweden) gradient centrifugation (Vereide et al., 2014). Peripheral blood mononuclear cells at a density of 1 × 10⁷ per mL were incubated with CD8‐coated magnetic microbeads (MiltenyiBiotec, Bergisch Gladbach, Germany) for 15 min at 4 °C. The cell suspension was loaded onto a MACS column placed in the magnetic field of a MACS separator. The magnetically labeled CD8⁺ T cells were retained within the column during washing with PBS. The CD8⁺ T cells were then eluted after removing the column from the magnetic field.