Cerulein

Manufactured by MedChemExpress

Sourced in United States, China

Cerulein is a laboratory equipment product used for research purposes. It is a synthetic peptide that structurally and functionally resembles the naturally occurring gastrointestinal hormone cholecystokinin. Cerulein can be utilized in various experimental settings to study physiological processes and pharmacological effects.

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Lab products found in correlation

Human recombinant epidermal growth factor, by Thermo Fisher Scientific (2 mentions) Kribb11, by MedChemExpress (2 mentions) Erlotinib, by MedChemExpress (2 mentions) Cerulein, by Merck Group (1 mentions) Dmxaa, by MedChemExpress (1 mentions) Anti il 17a, by BioXCell (1 mentions) Anti actin, by Proteintech (1 mentions) Anti tubulin, by Proteintech (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Amylase antibody, by Santa Cruz Biotechnology (1 mentions) Cd11b antibody, by Abcam (1 mentions) Moma 2 antibody, by Abcam (1 mentions) Zm 241385, by Selleck Chemicals (1 mentions) Gsk2606414, by Selleck Chemicals (1 mentions) Abt 702, by Merck Group (1 mentions) Grp78 antibody, by Proteintech (1 mentions) Chop antibody, by Proteintech (1 mentions) Perk antibody, by Proteintech (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Selumetinib, by MedChemExpress (1 mentions)

Close spelling variants of this product

Cerulein (HY-A0190)

8 protocols using cerulein

Murine Chronic Pancreatitis Model and MitoQ Treatment

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CP was induced via the repeated injection of mice with cerulein (Topscience, Shanghai, China) over a 4-week period (50 µg/kg) at 6-h intervals per day,3 times per week. cerulein is frequently used to establish murine CP models in this field.26 ,27 (link) Further, to evaluate the efficacy of MitoQ (MedChemExpress, Shanghai, China), mice in the MitoQ treatment group were administered MitoQ (MedChemExpress) via oral gavage daily (10 mg/kg) during the cerulein injection period. Finally, three days after the last cerulein (Topscience) injection, the mice were sacrificed, and pancreatic tissue samples were collected for observation.

Li M., Yuan Y., Han X., Liu X., Zhang W, & Hao J. (2022). Antioxidant Mitoquinone Alleviates Chronic Pancreatitis via Anti-Fibrotic and Antioxidant Effects. Journal of Inflammation Research, 15, 4409-4420.

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Cerulein-induced Chronic Pancreatitis Mouse Model

Cited 2 times

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Chronic pancreatitis was induced by repeated acute pancreatitis^{12 (link)}. In brief, sex and age matched mice (6-8 weeks old) received 6 hourly intraperitoneal injection of 50μg/kg cerulein (Sigma-Aldrich, St Louis, MO) 3 days/week for a total of 4 weeks. Mice were sacrificed 3 days after the last cerulein injection as described^{13 (link)}. For STING agonist treatment, mice were intraperitoneal injected with vehicle or 10mg/kg DMXAA (MedChem Express, New Jersey, USA) daily during the last 5 days of cerulein injection^{14 (link)}. For antibody neutralizing experiments, mice were treated with either isotype control or anti-mouse IL-17A (anti-IL-17A, 50μg/mouse/day, 3 times/week; Bio X Cell, NH, USA) antibodies during the last 2 weeks^{15 (link)}. To determine STING expression in leukocyte subsets (macrophages and Th17 cells) over time, mice receiving repeated cerulein or saline control (6 hourly injection/day, 3 days/week) were euthanized at week 1, 2, or 3.

Zhao Q., Manohar M., Wei Y., Pandol S.J, & Habtezion A. (2019). STING signaling protects against chronic pancreatitis by modulating Th17 response. Gut, 68(10), 1827-1837.

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Analyzing Cellular Lipid Metabolism

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The following commercial antibodies were used: anti-ASAH1 (rabbit, Abcam), anti-ASAH1 (mouse, Proteintech), anti-MIB1 (rabbit, Proteintech), anti-ubiquitination (rabbit, Proteintech), anti-actin (mouse, Proteintech), anti-tubulin (rabbit, Proteintech), and anti-ceramide (mouse, Thermo). Cerulein was obtained from MedChemExpress. LPS was from Sigma-Aldrich. Sphingosine was from MedChemExpress.

Xiao J., Zeng W., Zhang P., Zhou Y, & Fang Q. (2022). Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis. Molecular Medicine, 28, 106.

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Cerulein-Induced Pancreatitis Signaling

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Cerulein was purchased from MedChem Express (HY-A0190, Shanghai). Lipopolysaccharide (LPS) was purchased from Sigma (L2630, Shanghai). ABT702 was purchased from Merck Millipore (116890, Shanghai). ZM241385 (an adenosine A_2A receptor antagonist) and GSK2606414 (PERK inhibitor) were purchased from Selleck (S8105 and S7307, respectively, Shanghai). The following primary antibodies were purchased: ß-actin antibody (Proteintech, 66009), ADK antibody (Abcam, ab227087), RIP1 antibody (CST, 3,493), phosphor-RIP1 (Ser161) antibody (Affinity, AF7377), RIP3 antibody (CST, 15828), phosphor-RIP3 antibody (Abcam, ab195117), mouse MLKL antibody (Proteintech, 66675), rat MLKL antibody (Abcam, ab243142), phosphor-MLKL antibody (Affinity, AF7420), NF-κB p65 antibody (CST, 8242), phosphor-NF-κB p65 antibody (CST, 3,033), GRP78 Antibody (Proteintech, 11587), CHOP antibody (Proteintech, 15204), PERK antibody (Proteintech, 24390), phosphor-PERK antibody (CST, 3,179), eIF2α antibody (CST, 5324), phosphor-eIF2α antibody (CST, 3,398), CD11b antibody (Abcam, ab133357), MOMA-2 antibody (Abcam, ab33451), and amylase antibody (Santa Cruz Biotechnology, sc-46657). Secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, United States).

Sun S., Han Y., Zhang C., Liu H., Wang B., Cao S., Yuan Q., Wei S, & Chen Y. (2022). Adenosine Kinase Inhibition Prevents Severe Acute Pancreatitis via Suppressing Inflammation and Acinar Cell Necroptosis. Frontiers in Cell and Developmental Biology, 10, 827714.

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Cerulein-Induced Pancreatic Injury Model

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Cerulein (40 μg/kg or 5 μg/mouse), KRIBB11 (mainly 2 μM for cells and 50 mg/kg for mice), erlotinib (2 μM for cells and 100 mg/kg for mice), selumetinib (100 nM for cells) and torkinib (50 nM for cells) were purchased from MedChem Express (Monmouth Junction, NJ, USA). Recombinant human epidermal growth factor (EGF mainly 20 ng/ml for cells) and transforming growth factor alpha (mainly TGFα 50 ng/ml for cells) were purchased from PeproTech (Rocky Hill, NJ, USA). All reagents were stored according to the manufacturer’s instructions.

Qian W., Chen K., Qin T., Xiao Y., Li J., Yue Y., Zhou C., Ma J., Duan W., Lei J., Han L., Li L., Shen X., Wu Z., Ma Q, & Wang Z. (2021). The EGFR-HSF1 axis accelerates the tumorigenesis of pancreatic cancer. Journal of Experimental & Clinical Cancer Research : CR, 40, 25.

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Cerulein-Induced Acute Pancreatitis in Mice

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8-week-old C57BL/6 mice were pretreated with Nec-1 (1.66 mg/kg body weight) or Vemurafenib (5 mg/kg body weight) via intraperitoneal injection for 15 mins. Mice subsequently were injected intraperitoneally with saline or cerulein (MedChemExpress) at a concentration of 50 μg/kg body weight every 2 hours for 10 hours (total 6 injections). Mice were sacrificed after 2 hours of recovery. The pancreas was collected for H&E staining, and blood was collected for analysis of serum amylase and cytokines.

Sun M., Ma X., Mu W., Li H., Zhao X., Zhu T., Li J., Yang Y., Zhang H., Ba Q, & Wang H. (2023). Vemurafenib inhibits necroptosis in normal and pathological conditions as a RIPK1 antagonist. Cell Death & Disease, 14(8), 555.

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Vitamin A and Cerulein-Induced Pancreatitis

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Vitamin A palmitate (CAS NO. 79-81-2, Aladdin, China) was dissolved in corn oil and introduced by gavage or intraperitoneal injection. Cerulein (Stock NO. HY-A0190) was purchased from MedChemExpress, USA. Streptozocin (Code NO. S8050) was obtained from Solarbio, China.

Wang J., Li T., Zhou Y., Wang X., Carvalho V., Ni C., Li W., Wang Q., Chen Y., Shang Z., Qiu S, & Sun Z. (2023). Genetic lineage tracing reveals stellate cells as contributors to myofibroblasts in pancreas and islet fibrosis. iScience, 26(6), 106988.

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Cerulein, KRIBB11, and Erlotinib Protocol

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Cerulein (40 µg/kg or 5 µg/mouse), KRIBB11 (1,2 5, 10, 20 µM for cells and 50 mg/kg for mice), erlotinib (10 µM for cells and 100 mg/kg for mice) were purchased from MedChem Express (Monmouth Junction, NJ, USA). Recombinant human epidermal growth factor (EGF, 20 ng/ml for cells) was purchased from PeproTech (Rocky Hill, NJ, USA). All reagents were stored according to the manufacturer's instructions.

Qian W., Chen K., Qin T., Xiao Y., Li J., Yue Y., Zhou C., Ma J., Duan W., Lei J., Liang H., Shen X., Wu Z., Ma Q., & Wang Z. (2020). The EGFR-HSF1 Axis Accelerates the Tumorigenesis of Pancreatic Cancer.

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