5 5 dithiobis 2 nitrobenzoic acid dtnb trichloroacetic acid

Curcumin extract (90%) was purchased from Sisco Research Laboratories (SRL) Pvt. Ltd., (Mumbai, Maharashtra, India). High Capacity cDNA kit, phosphate buffer saline (PBS), formaldehyde, 5,5-dithiobis (2-nitrobenzoic acid) (DTNB), trichloroacetic acid, thiobarbituric acid (TBA), butanol, nitroblue tetrazolium (NBT), disodium hydrogen phosphate, sodium citrate, and hydrogen peroxide (H₂O₂) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Mouse brain-derived neurotrophic factor (BDNF) ELISA kit and mouse CREB ELISA kit were purchased from Ray Biotech (Georgia, GA, USA). Mouse Synapsin ELISA kit and mouse DCX ELISA kit were purchased from Gentaur (Kampenhout, Belgium). RNA Isolation Reagents and TRIzol were purchased from Sigma Aldrich (St. Louis, MO, USA). SYBR Green was purchased from Applied Biosystems, MA, USA. NanoDrop (NanoDrop Technologies, Wilmington, DE, USA), Polytron tissue homogenizer (Thomas Scientific, Swedesboro, NJ, USA), ELISA reader (Trans Asia Pvt. Ltd., Mumbai, Maharashtra, India), and UV spectrometer (Perkin Elmer, Waltham, MA, USA) were used during the conduction of experimental procedures.

Cadmium chloride and curcumin extract (95%) were purchased from Sisco Research Laboratory (SRL) Pvt. Ltd. (Pune, India). Phosphate buffer saline (PBS), pioglitazone, formaldehyde, 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), trichloroacetic acid, thiobarbituric acid (TBA), butanol, nitroblue tetrazolium (NBT), disodium hydrogen phosphate, sodium citrate, and hydrogen peroxide (H₂O₂) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Mouse IL-6, IL-10, and TNFα ELISA kits were purchased from Ray Biotech (Peachtree Corners, GA, USA). A polytron tissue homogenizer (Thomas Scientific, Swedesboro, NJ, USA), an ELISA reader (Trans Asia Pvt. Ltd., Mumbai, Maharashtra, India), and a UV spectrometer (Perkin Elmer, Waltham, MA, USA) were used during the experiment.