EV RNA extraction was performed using the miRCURY RNA Isolation Kit - Cell & Plant (#300110, Exiqon) following the manufacturer’s instructions. In brief, exosome pellet was lysed with the provided lysis solution supplemented with 96–100% ethanol, and the mixture was loaded to the column. The EV RNA was washed and eluted with 15–50 µL of RNase-free water. The extracted RNA concentration was calculated by Quant-IT RiboGreen (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA). RNA size was confirmed using Agilent RNA 6000 Pico Kit and Small RNA Kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany).
Agilent rna 6000 pico kit and small rna kit
Manufactured by Agilent Technologies
Sourced in Germany, United States
The Agilent RNA 6000 Pico Kit and Small RNA Kit are lab equipment used for the analysis of RNA samples. The Pico Kit is designed for the analysis of small amounts of RNA, while the Small RNA Kit is specifically for the analysis of small RNA molecules such as microRNA. Both kits utilize the Agilent 2100 Bioanalyzer system to provide automated, quantitative, and qualitative assessment of RNA samples.
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Lab products found in correlation
2 protocols using agilent rna 6000 pico kit and small rna kit
1
Exosomal RNA Extraction and Analysis
2
Optimized RNA Extraction for Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA, including miRNA, was isolated from tissue samples using miRNeasy Mini kit (Qiagen, Hilden, Germany). Tissues were homogenized (IKA Works, Staufen, Germany) with 700 µL QIAzol lysis buffer (Qiagen). Homogenates were processed according to the manufacturer’s instructions. RNA was eluted with RNase-free water. The integrity of the RNA was confirmed using Agilent RNA 6000 Pico Kit and Small RNA Kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
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