Flunixin meglumine banamine

SR95531 (selective GABA_A receptor antagonist) (TOCRIS, West-woods Business Park, Ellisville, MO, USA; cat. #1262), 2,2,2-tribromoethanol (Sigma-Aldrich, St Louis, Missouri, USA; cat. #T48402) and urethane (Sigma-Aldrich; cat. #U2500) were dissolved in saline (NaCl 0.9%). AM251 (N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlo-rophenyl)-4-methyl-1H-pyrazole-3 carboxamide) (selective CB₁ receptor antagonist) (TOCRIS, cat. #1117) was dissolved in a solution of saline containing 30% of DMSO (DMSO). Flunixin meglumine (Banamine, Schering Plough, Cotia, SP, Brazil) and the polyantibiotic preparation of streptomycins and penicillins (Pentabiotico, Fort Dodge, Campinas, SP, Brazil) were used as provided.

2-methyl-6-(phenylethynyl)-pyridine (MPEP) and 3-[(2-methyl-1,3-thiazol-4-yl) ethynyl] pyridine (MTEP) were purchased from Tocris Bioscience (Bristol, UK). Dimethyl sulfoxide (DMSO) and paraformaldehyde were purchased from Sigma Aldrich (St. Louis, MO, U.S.A.). Neutral red A.R. was purchased from Himedia Laboratories (Mumbai, MH, India). Glass microscope slides (25,4 mm x 76,2 mm) were from Global Glass (Beilun, ZHE, China). Sucrose was purchased from Synth (Diadema, SP, Brazil) and saline solution 0.9% (NaCl) from Equiplex (Aparecida de Goiânia, GO, Brazil). Ketamine hydrochloride and xylazine hydrochloride were purchased from Syntec (Cotia, SP, Brazil) and flunixin meglumine - Banamine® from Schering-Plough (Kenilworth, NJ, U.S.A). The zinc cement and dental acrylic were purchased from Coltene (São Jose, SC, Brazil) and cephalexin from Medley (Brasilia, DF, Brazil). The polyethylene tubing (PE20) was purchased from Tygon® Tubing (Ohio, U.S.A.).

Losartan (Sigma–Aldrich, St Louis, MO, USA), propranolol hydrochloride (Sigma–Aldrich), methylatropine (Sigma–Aldrich), phenylephrine hydrochloride (Sigma–Aldrich), sodium nitroprusside (Sigma–Aldrich), acetylcholine (Sigma–Aldrich), tribromoethanol (Sigma–Aldrich), and urethane (Sigma–Aldrich) were dissolved in saline (0.9% NaCl). Flunixin meglumine (Banamine^®; Schering-Plough, Cotia, SP, Brazil) and the poly-antibiotic preparation (Pentabiotico^®; Fort Dodge, Campinas, SP, Brazil) were used as provided.