Ba430 455

Manufactured by Olympus

The BA430-455 is a precision balance designed for laboratory use. It features a range of 430 grams and a readability of 0.01 grams. The balance is equipped with a backlit LCD display and offers basic weighing functions.

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Lab products found in correlation

Ba490 540, by Olympus (5 mentions) Ba575 620, by Olympus (4 mentions) Ba655 755, by Olympus (3 mentions) Xlumplanfl n 20x, by Olympus (2 mentions) Xlfluor, by Olympus (2 mentions) Uplansapo, by Olympus (2 mentions) Fv1000 confocal microscope, by Olympus (2 mentions) Ba575 675, by Olympus (2 mentions) Fv1000 confocal multiphoton imaging system, by Olympus (1 mentions) Xlumplfln 20x water immersion objective, by Olympus (1 mentions) Sdm473, by Olympus (1 mentions) Fluoview fv1000mpe, by Olympus (1 mentions) Sdm 640, by Olympus (1 mentions) Sdm560, by Olympus (1 mentions) Ix83 inverted microscope, by Olympus (1 mentions) Fv10 asw 4, by Olympus (1 mentions)

5 protocols using ba430 455

Intravital Microscopy of Tumor Cell Dynamics

Cited 1 time

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Intravital microscopy was performed on an Olympus FV1000 confocal-multiphoton imaging system using a XLUMPLFLN 20x water immersion objective (NA 1.0; Olympus America) with 2x digital zoom. Images were scanned sequentially using 405-nm, 473-nm, 559-nm, and 635-nm diode lasers with a DM405/473/559/635-nm dichroic beam splitter; emitted light was collected using combinations of beam splitters (SDM473, SDM560, and/or SDM 640) and emission filters BA430-455, BA490-540, BA575-620, and BA655-755 (all Olympus America).
Dorsal window chamber imaging was performed following previously described procedures (16 (link)); briefly, 2 million HT1080-membrane-mApple cells in 50 μl PBS were injected under the fascia of nu/nu mice (Cox7, MGH) 30 min after surgical chamber implantation and imaged two weeks later.

Miller M.A., Gadde S., Pfirschke C., Engblom C., Sprachman M.M., Kohler R.H., Yang K.S., Laughney A.M., Wojtkiewicz G., Kamaly N., Bhonagiri S., Pittet M., Farokhzad O.C, & Weissleder R. (2015). Predicting therapeutic nanoparticle efficacy using a companion MR imaging nanoparticle. Science translational medicine, 7(314), 314ra183.

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Intravital Imaging of IL-12 Induction

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Mice bearing dorsal window chambers with MC38-mTAG-BFP2 cells were imaged to determine the kinetics of IL-12 induction in the tumor microenvironment. Dorsal window chambers were implanted into mice using well-established techniques 37 (link). Fluorescent tumor cells (MC38-mTAGBFP2) were implanted in the window chambers as previously described 47 ,48 (link) and allowed to grow for 8-10 days before imaging experiments, with tumor growth monitored regularly.
All confocal images were collected using a customized Olympus FV1000 confocal microscope (Olympus America). A 2x (XLFluor, NA 0.14), a 4x (UPlanSApo, NA 0.16), and an XLUMPlanFL N 20x (NA 1.0) water immersion objective were used for imaging (Olympus America). Tumor cells (MC38-TagBFP2), fusion-protein IL-12-GFP, and CANDI^AF647 were excited sequentially using a 405 nm, a 473 nm, and a 633 nm diode laser in combination with a DM-405/488/559/635 nm dichroic beam splitter. Emitted light was further separated by beam splitters (SDM-473, SDM-560, and SDM-640) and emission filters BA430-455, BA490-540, BA575-620, and BA655-755 (Olympus America). Confocal laser power settings were carefully optimized to avoid photobleaching, phototoxicity, or tissue damage. Fiji (ImageJ, 2.3.0/1.54d) was used for image analysis.

Kim H.S., Halabi E.A., Enbergs N., Kohler R.H., Fei F., Garris C.S, & Weissleder R. (2024). A non-lipid nucleic acid delivery vector with dendritic cell tropism and stimulation. Theranostics, 14(7), 2934-2945.

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Intravital Imaging of Tumor PD-1 Dynamics

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Intravital microscopy was performed in dorsal skin-fold window chambers installed on DPE-GFP or GREAT mice inoculated with MC38-H2B-mApple tumors. Mouse macrophages and/or vasculature were labeled with Pacific Blue ferumoxytol and dextran, respectively. AF647-aPD-1 (200 μg) was delivered i.v. and its tumor distribution was observed using an Olympus FluoView FV1000MPE confocal imaging system (Olympus America), as described previously (44 (link)). Pacific Blue, GFP/YFP, mApple, and AF647 were imaged sequentially using 405, 473, 559, and 635 nm lasers and BA430-455, BA490-540, BA575-620, BA575-675 emission filters with DM473, SDM560, and SDM 640 beam splitters, all sourced from Olympus America. Time lapse images were acquired continually over the first hour following AF647-aPD-1 injection, after which the mice were allowed to recover before subsequent imaging.

Arlauckas S.P., Garris C.S., Kohler R.H., Kitaoka M., Cuccarese M.F., Yang K.S., Miller M.A., Carlson J.C., Freeman G.J., Anthony R.M., Weissleder R, & Pittet M.J. (2017). In vivo imaging reveals a tumor-associated macrophage mediated resistance pathway in anti-PD-1 therapy. Science translational medicine, 9(389), eaal3604.

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Intravital Imaging of Tumor Microenvironment

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Dorsal windows were implanted into IL-12 eYFP reporter mice.^{53 (link), 54 (link)} All confocal images were collected using a customized Olympus FV1000 confocal microscope (Olympus America). A 2x (XLFluor, NA 0.14), a 4x (UPlanSApo, NA 0.16), and an XLUMPlanFL N 20x (NA 1.0) water immersion objective were used for imaging (Olympus America). MC38 H2B-apple tumor cells, HAMT^AF647, and vascular probes were excited sequentially using a 405 nm, a 473 nm, a 559 nm, and a 633 nm diode laser, respectively, in combination with a DM-405/488/559/635 nm dichroic beam splitter. Emitted light was further separated by beam splitters (SDM-473, SDM-560, and SDM-640) and emission filters BA430-455, BA490-540, BA575-620, and BA655-755 (Olympus America). Confocal laser power settings were carefully optimized to avoid photobleaching, phototoxicity, or damage to the tissues. FIJI (ImageJ, 2.9.0/1.53t) was used for image analysis. HAMT^AF647 was administered as a single injection containing a mixture of HAMT 1+2+3 (5 mg per injection, 100 μL) and CANDI^AF647 (5 mg, 100 μL).

Harrod R, & Lovett P.S. (1995). Peptide inhibitors of peptidyltransferase alter the conformation of domains IV and V of large subunit rRNA: a model for nascent peptide control of translation.. Proceedings of the National Academy of Sciences of the United States of America, 92(19), 8650-8654.

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Thermosensitive Nanoparticle Imaging Protocol

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Fluorescence imaging was performed with an Olympus IX 83 inverted
microscope equipped with a FV12-FD camera (Olympus) and an oil immersion
objective lens (PLAPON 60×, NA = 1.42). The FV10-ASW 4.2 software
(Olympus) was used for controlling camera, filters, and recording
data. For dual color imaging of nanoHT, DM405/473 and
SDM473 were used as dichroic mirrors and BA430-455 and BA575-675 as
emission filters, respectively. For a tricolor imaging of nanoHT, DM405/473, SDM473, and SDM560, and BA430-455, BA490-540, and BA575-675
were used as dichroic mirrors and emission filters respectively (Olympus).
For photothermal stimulation during microscopic observation, an IR-LEGO
system (IR-LEGO-100/mini/E, SIGMAKOKI) was introduced to the microscopic
setup to allow laser stimulation at an 808 or 980 nm wavelength (100
mW). In the experiments using a 980 nm laser, iron oxide (Fe₂O₃) magnetic solution (5 μL) was allowed to dry
on a glass-based dish to be used as an external heat source. By illuminating
iron oxide particles with a 980 nm laser, the temperature gradient
was created during microscopic observation. To obtain the calibration
curve of nanoHT (normalized ratio of EuDT to C102 vs
temperature), the temperature in the medium was varied from 35 to
48 °C using a microscope temperature-controlled chamber (TOKAI-HIT).

F.e.r.d.i.n.a.n.d.u.s., Suzuki M., Vu C.Q., Harada Y., Sarker S.R., Ishiwata S., Kitaguchi T, & Arai S. (2022). Modulation of Local Cellular Activities using a Photothermal Dye-Based Subcellular-Sized Heat Spot. ACS Nano, 16(6), 9004-9018.

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