Oxo m

Manufactured by Merck Group

The Oxo-M is a versatile laboratory equipment designed for a range of applications. It functions as a multi-purpose stirrer and mixer, facilitating efficient sample preparation and homogenization. The Oxo-M operates through a stable, consistent rotational motion to ensure thorough mixing and agitation of various materials within a laboratory setting.

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Lab products found in correlation

Thapsigargin, by Thermo Fisher Scientific (2 mentions) Dgk inhibitor, by Bio-Techne (2 mentions) 1 2 dioleoyl 9 10 3h rac glycerol, by American Radiolabeled Chemicals (2 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions) 1 2 dioleoyl sn glycerol, by Avanti Polar Lipids (2 mentions) 1 2 dioleoyl sn glycero 3 phosphoserine, by Avanti Polar Lipids (2 mentions) Proteinase k, by Merck Group (2 mentions) Ionomycin, by Merck Group (2 mentions) Oxo m, by Avanti Polar Lipids (2 mentions) Ionomycin, by Avanti Polar Lipids (2 mentions) Thapsigargin, by Avanti Polar Lipids (2 mentions) Dgk inhibitor, by Avanti Polar Lipids (2 mentions) 3h rx821002, by PerkinElmer (1 mentions) Phospho erk1 2, by Santa Cruz Biotechnology (1 mentions) N methyl 3h scopolamine methyl chloride 3h nms, by PerkinElmer (1 mentions) Calregulin, by Santa Cruz Biotechnology (1 mentions) Antibodies against erk1 2, by Cell Signaling Technology (1 mentions) Uk14304, by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

4 protocols using oxo m

Biochemical Reagents for Cell Signaling

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Antibodies against GFP, human Rab43, calregulin, phospho-ERK1/2, and β-actin were purchased from Santa Cruz Biotechnology. Antibodies against ERK1/2 were from Cell Signaling Technology. BFA, UK14304, and Oxo-M were obtained from Sigma-Aldrich. The radioligands [³H]-RX821002 (50 Ci/mmol) and [N-methyl-³H]-scopolamine methyl chloride ([³H]-NMS, 80 Ci/mmol) were from PerkinElmer Life Sciences. All other materials were obtained as described elsewhere (63 (link)).

Wei Z., Xu X., Fang Y., Khater M., Naughton S.X., Hu G., Terry AV J.r, & Wu G. (2021). Rab43 GTPase directs postsynaptic trafficking and neuron-specific sorting of G protein–coupled receptors. The Journal of Biological Chemistry, 296, 100517.

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Lipid Reagents for Cell Signaling

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Primary and secondary antibodies used in this study are listed in Supplementary Table 1. Oxo-M, Atropine and Ionomycin (Sigma-Aldrich). Thapsigargin (Invitrogen/Life-technologies). DGK inhibitor (R 59–022, Tocris Bioscience). Proteinase K (Sigma-Aldrich). Following concentration of chemicals are used in all the experiments unless noted: Oxo-M, 10μM; Atropine, 50μM; Ionomycin, 2μM; Thapsigargin, 2μM; DGK inhibitor, 50μM. All non radiolabeled lipids were obtained from Avanti Polar Lipids; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 850457; 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), 840035; L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P₂), 840046; (NBD)-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 810144; 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] (DGS-NTA(Ni)), 790404; 1-2-dioleoyl-sn-glycerol (DAG), 800811. Radiolabeled lipids were purchased from American Radiolabeled Chemicals (St. Louis, MO); 1,2-Dioleoyl [9,10-3H] rac-glycerol, ART 2185.

Saheki Y., Bian X., Schauder C.M., Sawaki Y., Surma M.A., Klose C., Pincet F., Reinisch K.M, & De Camilli P. (2016). Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nature cell biology, 18(5), 504-515.

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Lipid Reagents for Cell Signaling

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Capsaicin-Induced C-Fiber Desensitization

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To induce C-ber desensitization, capsaicin (125 mg/kg, dissolved in 10% ethanol, 10% Tween 80 and 80% physiological saline) was injected subcutaneously into rats. As described in previous studies [29] [30] [31] , capsaicin was administered in divided doses on 2 consecutive days: 25 and 50 mg/kg at a 12-h interval on the 1st day and 50 mg/kg on the 2nd day, and the experiments were performed 4 days after the last injection. Oxo-M (Sigma; dissolved in saline; intraperitoneal injection of 0.2 mg/kg), a preferential M2 agonist [32, 33] , was administrated at 15 minutes before each moxibustion treatment. Metho, a selective M2 receptor antagonist (Sigma; dissolved in saline; intraperitoneal injection of 0.3 mg/kg) [34] , was used in our experiments.

Wang L., Fu Y.B., Liu Y., Yang N.N., Ma S.M., Wang X.R., Huang J., Shi G.X., Yang J.W, & Liu C.Z. (2022). Moxibustion attenuates neurogenic detrusor overactivity in spinal cord injury rats by inhibiting M2/ATP/P2X3 pathway. Brain research, 1788.

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