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Ftir 8700

Manufactured by Shimadzu
Sourced in Japan

The FTIR-8700 is a Fourier Transform Infrared (FTIR) spectrometer manufactured by Shimadzu. It is a versatile analytical instrument used for the identification and analysis of various materials through infrared spectroscopy. The FTIR-8700 utilizes advanced optical and electronic components to provide high-performance spectroscopic data.

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6 protocols using ftir 8700

1

Spectroscopic Characterization of Pigments

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The UV-Vis absorption range of pigments was determined by performing their spectral analysis using UV-Vis spectrophotometry (Epoch 2 microplate spectro-photometer). The pigments solubilized in 5% methanol were analyzed using methanol as blank. The chemical environments of the extracted pigments were determined by FTIR spectroscopy (FTIR 8700, Shimadzu Co., Tokyo, Japan). The samples (10 mg) were mixed homogenously with dried crystals of KBr (200 mg) to make pellets and immediately used for the FTIR analysis (in the range of 4000 to 400 cm−1) to avoid any water absorption.
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2

Spectroscopic Characterization of Organic Compounds

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Optical rotations were recorded on a JASCO P-2200 digital polarimeter. Infrared spectra (IR) were measured with a Shimadzu FTIR-8700 instrument for samples in CHCl3. NMR spectra were taken on a JEOL JMN-ECA600 spectrometer and JMN-ECS400 spectrometer with tetramethylsilane as an internal standard, and chemical shifts are expressed as δ values. High-resolution mass spectrometric data were obtained on a JEOL JMS-700 (FAB) or a JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC was carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed with silica gel and C18 cartridges (Biotage, Uppsala, Sweden).
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3

Characterization of Synthetic Compounds

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Optical rotations were recorded on a JASCO P-2200 digital polarimeter. Infrared spectra (IR) were measured with a Shimadzu FTIR-8700 instrument for samples in CHCl3. NMR spectra were acquired on JEOL JMN-ECA600 and JMN-ECS400 spectrometers with tetramethylsilane as internal standard, and chemical shifts are expressed as δ values. HRMS data were obtained on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Microwave irradiation experiments were carried out in a dedicated Biotage Initiator 2.5 microwave apparatus. Analytical and preparative TLC was carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed with silica gel and C18 cartridges (Biotage, Uppsala Sweden). Compounds 91316 ,17 (link),20 and 1921 (link) were obtained previously.
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4

Characterization of Organic Compounds

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Optical rotations were recorded on a JASCO P-2200 digital polarimeter. Infrared spectra (IR) were measured with a Shimadzu FTIR-8700 instrument for samples in CHCl3. NMR spectra were recorded on JEOL JMN-ECA600 and JMN-ECS400 spectrometers with tetramethylsilane as an internal standard, and chemical shifts are expressed as δ values. HRMS data were obtained on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC were carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed with silica gel and C18 cartridges (Biotage, Uppsala Sweden).
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5

Characterization of HAsPBPE Conjugates

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The synthesis of HAsPBPE conjugates was confirmed with the 1H 500 MHz superconducting Fourier transform-nuclear magnetic resonance (NMR) spectroscopy (Varian Unity Inova; Varian Inc., Santa Clara, CA, USA).
The Fourier transform-infrared (FT-IR) spectroscopy (FT-IR 8700; Shimadzu, Osaka, Japan) was also used to confirm the synthesis of conjugates.
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6

Characterization of Silver Nanoparticle-VAM Interactions

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Absorption spectra of the AgNPs and AgNP-VAM were obtained by UV-visible (UV-vis) spectroscopy (S31500; SCINCO, Seoul, Korea). The size of the nanoparticles was measured with a Zetasizer (Nano-ZS; Malvern, Malvern, UK). Fourier transform infrared spectrophotometry (FTIR 8700; Shimadzu, Kyoto, Japan) was used to confirm the chemical bonding between the VAM and AgNPs. Morphological analysis of the nanoparticles was carried out by confocal laser scanning microscopy (LSM 700; Carl Zeiss Microimaging GmbH, Jena, Germany) and scanning transmission electron microscopy (STEM; JEM, Akishima, Japan). Samples for TEM studies were prepared by placing one drop of the AgNP and AgNP-VAM solutions on carbon-coated TEM grids (Okenshoji, Tokyo, Japan), drying the grids at room temperature, and observing them at 150K× magnification in the microscope operated at 120 kV. The optical density of the M. smegmatis cells was investigated by UV-vis spectrophotometry (UV Mini 1240; Shimadzu, Kyoto, Japan) at 600 nm. Samples were diluted if the optical density exceeded the measurement capability of the spectrophotometer.
Quantification of the biomaterial-treated M. smegmatis was carried out by counting the viable colonies on LB agar plates and was expressed as colony-forming units per milliliter (CFU/mL).
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