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Ec plan neofluar 100 1.3 oil m27 objective

Manufactured by Zeiss
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The EC Plan-Neofluar 100×/1.3 Oil M27 objective is a high-magnification lens designed for use in advanced microscopy applications. It features a numerical aperture of 1.3 and is optimized for oil immersion, allowing for high-resolution imaging. The objective is part of the Zeiss EC Plan-Neofluar series, known for its advanced optical performance.

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Lab products found in correlation

Axio observer z1, by Zeiss (3 mentions) Digital ccd camera, by Hamamatsu Photonics (2 mentions) Axio imager m1 microscope, by Hamamatsu Photonics (2 mentions) Ti2e microscope, by Yokogawa (1 mentions)

Close spelling variants of this product

× 100/1.3 oil EC Plan-Neofluar objective EC Plan-Neofluar 100×/1.3 oil Plan-Neofluar 100×/1.3 Oil objective EC Plan-Neofluar objective EC Plan-Neofluar Plan-Neofluar objective 100× (1.3 oil) objective Plan-Neofluar Neofluar objectives

3 protocols using ec plan neofluar 100 1.3 oil m27 objective

1

High-resolution Live Cell Imaging

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Cells were imaged at room temperature in a 96-well plate with a glass bottom (655892; Greiner Bio-One) using an inverted microscope Zeiss Axio Observer Z1 equipped with an EC Plan-Neofluar 100×/1.3 Oil M27 objective, an EC Plan-Neofluar 40×/1.3 Oil RMS, an Orca Flash 4.0 LT+ camera, and a ZEN blue 3.3 pro software on Windows 11 pro 64-bit. Alternatively, experiments were performed with an inverted Nikon Ti2-E microscope (RRID:SCR_021068) equipped with a Yokogawa CSU-X1-A1 Nipkow spinning disk, a CFI Plan Apo λ 100×/1.45 Oil, WD 0.13 mm objective, a sCMOS back-illuminated Teledyne Prime BSI camera, and a VisiView 6.0 (Visitron Systems) software running on Windows 10 (64 bit). Images were deconvolved with Huygens Professional 23.04 (Scientific Volume Imaging) and analyzed using Fiji Version 2.9.0/1.53t (RRID:SCR_002285).
Baumann V., Achleitner S., Tulli S., Schuschnig M., Klune L, & Martens S. (2024). Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activation. The Journal of Cell Biology, 223(7), e202309057.
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2

Immobilizing Cells on Agarose Pads

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Cells were immobilized on agarose pads (1% w/v in reverse osmosis-purified water). Images were taken using a Zeiss EC Plan-Neofluar 100×/1.3 Oil M27 objective on a Zeiss AxioImager M1 microscope with a Hamamatsu Digital CCD Camera (C8484-03G01). Images were acquired using iVision-Mac software (BioVision Technologies) and processed using ImageJ (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, https://imagej.nih.gov/ij/, 1997–2018).
Zik J.J., Yoon S.H., Guan Z., Skidmore G.S., Gudoor R.R., Davies K.M., Deutschbauer A.M., Goodlett D.R., Klein E.A, & Ryan K.R. (2022). Caulobacter lipid A is conditionally dispensable in the absence of fur and in the presence of anionic sphingolipids. Cell reports, 39(9), 110888.
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3

Immobilizing Cells on Agarose Pads

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were immobilized on agarose pads (1% w/v in reverse osmosis-purified water). Images were taken using a Zeiss EC Plan-Neofluar 100×/1.3 Oil M27 objective on a Zeiss AxioImager M1 microscope with a Hamamatsu Digital CCD Camera (C8484-03G01). Images were acquired using iVision-Mac software (BioVision Technologies) and processed using ImageJ (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, https://imagej.nih.gov/ij/, 1997–2018).
Zik J.J., Yoon S.H., Guan Z., Skidmore G.S., Gudoor R.R., Davies K.M., Deutschbauer A.M., Goodlett D.R., Klein E.A, & Ryan K.R. (2022). Caulobacter lipid A is conditionally dispensable in the absence of fur and in the presence of anionic sphingolipids. Cell reports, 39(9), 110888.
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