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Ab37477

Manufactured by Abcam
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Sourced in United Kingdom

Ab37477 is a laboratory reagent. It is a recombinant protein product.

Automatically generated - may contain errors

Lab products found in correlation

Sc 47778, by Santa Cruz Biotechnology (2 mentions) Ab4729, by Abcam (2 mentions) Ab1791, by Abcam (2 mentions) Ab70469, by Abcam (2 mentions) Ab8898, by Abcam (2 mentions) Alexa fluor 680 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Ab992, by Merck Group (1 mentions) Odyssey clx, by LI COR (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Abe24, by Merck Group (1 mentions) R960 25, by Thermo Fisher Scientific (1 mentions) F7425, by Merck Group (1 mentions) M11217, by Thermo Fisher Scientific (1 mentions) Ab992, by Abcam (1 mentions)

3 protocols using ab37477

1

Chromatin Remodeling Complex Protein Detection

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Primary antibodies for Western blots used were rabbit anti-human SNF2H (Bethyl Laboratories, A301-081A), mouse anti-CTCF (Abcam, ab37477), rabbit anti-RAD21 (Abcam, ab992), rabbit anti-CHD1 (Bethyl Laboratories, A301-218A), rabbit anti-CHD2 (Active Motif, 39364), rabbit anti-CHD4 (Bethyl Laboratories, A301-081A), rabbit anti-ACF1 (Bethyl Laboratories, A301-318A), rabbit anti-WSTF (Cell Signaling, 2152), rabbit anti–TIP5 (Invitrogen, 491037) and mouse anti-beta actin (Sigma, A2228). Primary antibodies for ChIP used were rabbit anti-human SNF2H (Abcam, ab72499), mouse anti-CTCF (Millipore, 17–10044), rabbit anti-RAD21 (Abcam, ab992), rabbit anti-BPTF (Millipore, Abe24), and rat anti-SNF2L (2C4, [39 (link)] which has been kindly provided by P. Becker). Secondary antibodies for Western blots used were Alexa Fluor 680 goat anti-mouse (Invitrogen) and Alexa Fluor 790 goat anti-rabbit (Invitrogen) for immunofluorescence staining and analysis using the LI-COR Odyssey CLx.
Wiechens N., Singh V., Gkikopoulos T., Schofield P., Rocha S, & Owen-Hughes T. (2016). The Chromatin Remodelling Enzymes SNF2H and SNF2L Position Nucleosomes adjacent to CTCF and Other Transcription Factors. PLoS Genetics, 12(3), e1005940.
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2

Chromatin Immunoprecipitation Assay for Transcription Factors

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Antibodies against CHD4 (ab70469; Abcam [UK] and in-house generated), CTCF (ab37477; Abcam), α-tubulin (2144S; Cell Signaling Technology, USA), V5 (R960-25; Thermo Fisher Scientific), mCherry2 (M11217; Invitrogen), β-actin (SC-47778; Santa Cruz Biotechnology), and FLAG (F7425; Sigma) were used for immunoblotting. Antibodies against CHD4 (ab70469; Abcam and in-house generated) and IgG (12-371; Millipore) were used for immunoprecipitation. Antibodies against CTCF (07-729; Millipore), RAD21 (ab992; Abcam), H3 (ab1791; Abcam), H3K9me3 (ab8898; Abcam), H3K4me1 (in-house generated), H3K4me3 (in-house generated), H3K27ac (ab4729; Abcam), and H3K27me3 (07-449; Millipore) were used for ChIP-seq. Antibodies against CHD4 (ab70469; Abcam and in-house generated), RAD21 (ab992; Abcam), and IgG (12-371; Millipore and in-house generated) were used for CUT&RUN.
Han S., Lee H., Lee A.J., Kim S.K., Jung I., Koh G.Y., Kim T.K, & Lee D. (2021). CHD4 Conceals Aberrant CTCF-Binding Sites at TAD Interiors by Regulating Chromatin Accessibility in Mouse Embryonic Stem Cells. Molecules and Cells, 44(11), 805-829.
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3

ChIP-seq and Immunoblotting Protocol

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Antibodies against Chd4 (ab70469, Abcam), CTCF (ab37477, Abcam), and β-actin (SC-47778, Santa Cruz Biotech) were used for immunoblotting. Antibodies against Chd4 (ab70469, Abcam and inhouse generated) and IgG (12-371, Millipore) were used for immunoprecipitation. Antibodies against CTCF (07-729, Millipore), H3 (ab1791, Abcam), H3K9me3 (ab8898, Abcam), H3K4me1 (in-house generated), H3K4me3 (in-house generated), H3K27ac (ab4729, Abcam), and H3K27me3 (07-449, Millipore) were used for ChIP-seq.
Han S., Lee H., Lee A., Kim S., Jung I., Koh G.Y., Kim T., & Lee D. (2021). CHD4 conceals aberrant CTCF-binding sites at TAD interiors by regulating chromatin accessibility in mESCs.
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