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Ac160ts r3 cantilevers

Manufactured by Olympus
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The AC160TS-R3 cantilevers are a product offered by Olympus. They are designed for use in atomic force microscopy (AFM) applications. The cantilevers feature a silicon nitride tip and are suitable for tapping mode and contact mode measurements. The specific dimensions and specifications of the cantilevers are not available in this factual and unbiased description.

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Lab products found in correlation

Photonic professional gt 3, by Nanoscribe (2 mentions) Pgmea developer solution, by MicroChem (2 mentions) Autocad, by Autodesk (2 mentions) Igor pro, by Wavemetrics (2 mentions) Mfp 3d bio, by Olympus (1 mentions) Mfp 3d bio, by Oxford Instruments (1 mentions)

Close spelling variants of this product

AC160TS-R3 AC160TS cantilevers AC160TS Cantilevers

4 protocols using ac160ts r3 cantilevers

1

Fabrication of Fibronectin Nanofibers via 3D Printing

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To fabricate FN nanofibers with a submicron width, we used a Photonic Professional GT 3D printer (Nanoscribe GmbH). Briefly, a master mold containing 250 µm long, 5 µm wide, 2 µm high, and 500 nm spaced features was first designed in AutoCAD (Autodesk Inventor) and exported into DeScribe 2.0 software. The master mold was then 3D printed using IP-L photoresist. Unexposed regions were then removed by immersion in a PGMEA developer solution (Microchem). PDMS stamps containing the negative of the master mold (yielding 500 nm wide features) were prepared by casting the PDMS pre-polymer solution over the master mold as previously mentioned. To accurately assess their nanoscale dimensions, the FN was µCP onto PIPAAm-coated glass coverslips and scanned using AFM in air using AC mode with AC160TS-R3 cantilevers (Olympus Corporation). High-resolution AFM images were obtained using a scan size of 1024×1024 lines over a scan area of 8 µm×8 µm. The nanofiber width was quantified using the IGOR Pro software environment (WaveMetrics, Inc.).
Sun Y., Jallerat Q., Szymanski J.M, & Feinberg A.W. (2014). Conformal Nanopatterning of Extracellular Matrix Proteins onto Topographically Complex Surfaces. Nature methods, 12(2), 134-136.
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2

Atomic Force Microscopy Tapping Mode

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Samples were tested with Asylum MFP-3D-Bio using the tapping mode with AC160TS-R3 cantilevers (Olympus, k ≈ 26 N/m, ν ≈ 300 kHz). The data are presented in Fig. 2B and figs. S1B, S2C, and S8A.
Li Y., Li K., Wang X., Cui M., Ge P., Zhang J., Qiu F, & Zhong C. (2020). Conformable self-assembling amyloid protein coatings with genetically programmable functionality. Science Advances, 6(21), eaba1425.
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3

Fabrication of Fibronectin Nanofibers via 3D Printing

Check if the same lab product or an alternative is used in the 5 most similar protocols
To fabricate FN nanofibers with a submicron width, we used a Photonic Professional GT 3D printer (Nanoscribe GmbH). Briefly, a master mold containing 250 µm long, 5 µm wide, 2 µm high, and 500 nm spaced features was first designed in AutoCAD (Autodesk Inventor) and exported into DeScribe 2.0 software. The master mold was then 3D printed using IP-L photoresist. Unexposed regions were then removed by immersion in a PGMEA developer solution (Microchem). PDMS stamps containing the negative of the master mold (yielding 500 nm wide features) were prepared by casting the PDMS pre-polymer solution over the master mold as previously mentioned. To accurately assess their nanoscale dimensions, the FN was µCP onto PIPAAm-coated glass coverslips and scanned using AFM in air using AC mode with AC160TS-R3 cantilevers (Olympus Corporation). High-resolution AFM images were obtained using a scan size of 1024×1024 lines over a scan area of 8 µm×8 µm. The nanofiber width was quantified using the IGOR Pro software environment (WaveMetrics, Inc.).
Sun Y., Jallerat Q., Szymanski J.M, & Feinberg A.W. (2014). Conformal Nanopatterning of Extracellular Matrix Proteins onto Topographically Complex Surfaces. Nature methods, 12(2), 134-136.
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4

AFM Imaging of Laminin Nanofibers

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Laminin nanofibers were imaged in the pre- and post-release states using AFM (MFP-3D-BIO, Asylum Research) in AC mode in air with AC160TS-R3 cantilevers (Olympus Corporation). For AFM imaging, low resolution scans were first performed with a scan size of 1024×1024 points and lines over a scan area of 16 μm × 32 μm area for the Lam95 fiber pre-release and a scan area of 30 μm × 90 μm for the Lam95 fiber post-release. To resolve the nanostructure, the scan size was kept at 1024×1024 points and lines but the scan area was reduced to 1.5 μm × 1.5 μm and 4.0 μm × 4.0 μm for the Lam95 fibers pre- and post-release, respectively. Measurement of Lam95 nanofiber thickness was performed in the Igor Pro software environment. Briefly, the AFM height channel was first processed to flat to ensure the background was flat and centered at 0 nm. This was accomplished by applying a mask on the Lam95 nanofiber and subsequently subjecting the non-masked regions to a 1st order flattening. Once the background was appropriately flattened and centered at 0 nm, the thickness of the nanofibers could be measured.
Szymanski J.M., Ba M, & Feinberg A.W. (2015). Spontaneous Helical Structure Formation in Laminin Nanofibers. Journal of materials chemistry. B, Materials for biology and medicine, 3(40), 7993-8000.
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