Cells were lysed with radio immunoprecipitation assay buffer (RIPA) containing 1 mmol/L PMSF (Solarbio, China). Protein extractions were separated by 12% SDS-PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Germany). The membranes were incubated overnight at 4 °C with primary antibodies specific for anti-β-actin, anti-CDK2, anti-CyclinD1, anti-CyclinE, anti-PCNA, and anti-P53 (1:1,000; Wanleibio), anti-Bcl-2, anti-Bax, anti-Caspase9, anti-FOXC1, and anti-MyoD1 (1:1,000; Abways Technology), anti-MyoG, (1:1,000; Abcam, Cambridge, England). After incubation with secondary antibodies, signals were detected using a chemiluminescence system (Bio-Rad, USA).
Anti caspase9
Manufactured by Abways
Sourced in United Kingdom
Anti-Caspase9 is a lab equipment product that functions as a specific inhibitor of the apoptosis-related enzyme Caspase-9.
Automatically generated - may contain errors
Lab products found in correlation
Chemiluminescence system, by Bio-Rad (1 mentions)
Anti myog, by Abcam (1 mentions)
Anti cdk2, by Wanlei (1 mentions)
Anti cycline, by Wanlei (1 mentions)
Anti pcna, by Wanlei (1 mentions)
Anti p53, by Wanlei (1 mentions)
Anti bax, by Abways (1 mentions)
Anti β actin, by Wanlei (1 mentions)
Anti cyclin d1, by Wanlei (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions)
Anti bcl 2, by Wanlei (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Santa Cruz Biotechnology (1 mentions)
β actin, by Proteintech (1 mentions)
2 protocols using anti caspase9
1
Western Blot Analysis of Cell Signaling
2
Apoptosis Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The Western blot assay was conducted as previously described.22 (link) The primary antibodies were as follows: anti-Bcl-2 (Santa Cruz), anti-Bax (Cell Signaling Technology), anti-Caspase-9 (Abways), anti-Caspase-7 (Abways), and β-actin (Proteintech). The loading control was β-actin.
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