Goat anti rabbit immunoglobulin g igg
Goat-anti-rabbit immunoglobulin G (IgG) is a secondary antibody produced in goats that specifically binds to rabbit IgG antibodies. It is used in various immunoassay techniques to detect and quantify the presence of rabbit IgG in samples.
Lab products found in correlation
5 protocols using goat anti rabbit immunoglobulin g igg
Quantification of Gag.MS2 Protein Levels
Immunohistochemical Analysis of Lubricin, RSPOs, and LGRs
Lubricin, RSPOs and LGRs were immune stained using
the two-step immunohistochemistry procedure according
to the manufacturer’s protocol (Santa Cruz Biotechnology,
USA). In short, the tissue sections were treated with
rabbit polyclonal antibody for lubricin (Santa Cruz Immunohistochemistry
Lubricin, RSPOs and LGRs were immune stained using
the two-step immunohistochemistry procedure according
to the manufacturer’s protocol (Santa Cruz Biotechnology,
USA). In short, the tissue sections were treated with
rabbit polyclonal antibody for lubricin (Santa Cruz Biotechnology, USA), RSPO1 (Sigma-Aldrich, USA),
RSPO2 (Sigma-Aldrich, USA), LGR5 (Sigma-Aldrich,
USA), LGR6 (Abcam, England) and β-catenin (Santa
Cruz Biotechnology, USA) of 1:500 dilutions at 4˚C.
The slides were washed thrice with phosphate-buffered
saline (PBS, Wel Gene, Korea) and goat anti-rabbit
immunoglobulin G (IgG, Santa Cruz Biotechnology,
USA) was treated at room temperature for 30 minutes.
Western blot bands were developed for visualization with
3, 3′-diaminobenzidine as the chromogen. Each section
was photographed at ×10 magnification by a Zeiss Axio
Cam digital camera.
Unfolded Protein Response Pathway Analysis
Notch Pathway Activation in GSCs
Immunohistochemical Localization of Lubricin in Osteoarthritic Femoral Heads
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