The pharmacokinetic study was carried out in healthy male volunteers (n=6). Blood samples were collected in K2-EDTA vacutainer collection tubes (BD, Franklin, NJ, USA) following oral administration of esomeprazole (40 mg), rabeprazole (20 mg) and levosulpiride (75 mg) at pre-dose and 0.25, 0.50, 1.00, 1.50, 2, 2.5, 3, 4, 5, 6, 8, 10, 12, 16 and 24 h. The tubes were centrifuged (Thermo Scientific, Germany) at 3200 rpm for 10 min and then the plasma was collected. The collected plasma samples were stored at −80 °C till use. This study was carried out as per the approval and guidelines of the local ethical committee. Plasma samples were spiked with the IS and processed as per the extraction procedure described earlier. Along with the clinical samples, the QC samples at low, middle and high concentration levels were also assayed in triplicate. Plasma concentration vs time profiles of esomeprazole, rabeprazole and levosulpiride was analyzed by non-compartmental method using WinNonlin Version 5.3 (Pharsight Corporation, CA, USA).
K2 edta vacutainer collection tubes
Manufactured by BD
Sourced in United States
K2-EDTA vacutainer collection tubes are used to collect and store blood samples. They contain the anticoagulant K2-EDTA, which prevents the blood from clotting during the collection and storage process.
Automatically generated - may contain errors
Lab products found in correlation
Oncobeam ras crc assay, by Sysmex (2 mentions)
Winnonlin version 5, by Pharsight (1 mentions)
Multiplex oligos, by New England Biolabs (1 mentions)
Bioruptor pico, by Diagenode (1 mentions)
Gentra puregene blood kit, by Qiagen (1 mentions)
Nextseq, by Illumina (1 mentions)
Cell free dna bct collection tubes, by Streck (1 mentions)
Sepmate pbmc isolation tube, by STEMCELL (1 mentions)
Sfbs au, by Bovogen (1 mentions)
Lymphoprep, by STEMCELL (1 mentions)
Colorimetric assay kit, by Cayman Chemical (1 mentions)
Vacutainer, by BD (1 mentions)
7 protocols using k2 edta vacutainer collection tubes
1
Pharmacokinetic Study of Esomeprazole, Rabeprazole and Levosulpiride
2
Rhesus Macaque Whole Genome Sequencing
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Whole blood was obtained from the male and female rhesus macaque parents in K2EDTA vacutainer collection tubes (BD Diagnostics) by the Colony Genetics Resource Core within the Primate Genetics Program at Oregon National Primate Research Center (ONPRC). Parental DNA was extracted using the Gentra Puregene blood kit (Qiagen) according to the manufacturer's protocol and stored at −80°C. Samples (1 µg) were fragmented using the Diagenode Bioruptor pico for a 300- to 400-bp size selection. The NEBNext DNA library prep master mix set and multiplex oligos (NEB) were then used to generate Illumina whole-genome sequencing libraries. Libraries were quantified with the Qubit HS DNA kit and size distribution assessed with the 2100 Bioanalyzer. Multiplexed libraries were sequenced at the Oregon State University Center for Genomic Research and Biocomputing on the HiSeq 3000 platform using the 150 paired-end protocol for a total of 2.84 × 109 reads (1.56 × 108 reads/sample). One parental sample (ID: 26129) was sequenced on the Illumina NextSeq using our custom 75-bp paired-end protocol for a total of approximately 3.50 × 108 reads.
3
Topical Lidocaine-Prilocaine Absorption Kinetics
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Lidocaine (2.5%) and prilocaine (2.5%) cream was applied topically after observing overnight fast of at least 10 hours. Lidocaine and prilocaine cream 50 g was applied dermally over 400 cm2 on thigh for 4 hours at which time the excess drug was removed. Cream was applied within 5 min of specified drug administration time. Blood samples (4 mL) were collected before application of the cream and at 1, 2, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 9, 10, 12, 15, 18, 24 and 36 h after application in K2-EDTA vacutainer collection tubes (BD, Franklin, NJ, USA). The tubes were centrifuged to collect the plasma at 3200rpm for 10 min at 4 °C. the subject plasma samples were stored at −70 ± 5 °C until their analysis. Plasma concentration-time profiles of lidocaine and prilocaine were analyzed using WinNonlin software Version 5.1 by non-compartmental model.
An incurred sample re-analysis (ISR) was also conducted, selecting the seven subject samples (2 samples from each subject) near Cmax and the elimination phase. The variability (% difference) between original value and ISR values were within ±20%.
An incurred sample re-analysis (ISR) was also conducted, selecting the seven subject samples (2 samples from each subject) near Cmax and the elimination phase. The variability (% difference) between original value and ISR values were within ±20%.
4
Longitudinal Monitoring of ctDNA in Cancer
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Blood samples for ctDNA analysis were collected prior to starting first-line treatment (T0), 4 (T1), 8 weeks (T2) after starting treatment, and every 12 weeks thereafter (T3……n) until disease progression (T PD), as shown in Figure 1 . A plasma sample was also collected at the time of radiological progression according to RECIST version 1.1 criteria [28 (link)].
For each patient enrolled in the study, 8 mL of peripheral blood was collected in either K2 EDTA BD Vacutainer® collection tubes (BD, Franklin Lakes, NJ, USA) or Cell Free DNA BCT collection tubes (Streck, La Vista, NE, USA) by the nurses of Medical Oncology units of the abovementioned hospitals, and this was taken immediately before starting therapy. Plasma was then prepared within 4 or 72 h, depending on the collection tubes used, and according to the protocol released by Sysmex-Inostics for the determination of KRAS and NRAS status with OncoBEAM® RAS CRC assay (Sysmex Inostics, Hamburg, Germany). Plasma samples were stored at −80 °C.
For each patient enrolled in the study, 8 mL of peripheral blood was collected in either K2 EDTA BD Vacutainer® collection tubes (BD, Franklin Lakes, NJ, USA) or Cell Free DNA BCT collection tubes (Streck, La Vista, NE, USA) by the nurses of Medical Oncology units of the abovementioned hospitals, and this was taken immediately before starting therapy. Plasma was then prepared within 4 or 72 h, depending on the collection tubes used, and according to the protocol released by Sysmex-Inostics for the determination of KRAS and NRAS status with OncoBEAM® RAS CRC assay (Sysmex Inostics, Hamburg, Germany). Plasma samples were stored at −80 °C.
5
Plasma-based RAS mutation assessment
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Peripheral blood (8 ml) was collected from each patient enrolled in the study in K2 EDTA BD Vacutainer® collection tubes (BD Biosciences) immediately before starting therapy. The plasma was isolated within 4 h according to the Sysmex-Inostics protocol for the determination of KRAS and NRAS status with the OncoBEAM® RAS CRC assay (Sysmex Inostics). The plasma samples were stored at −80°C.
6
Blood Sample Processing for PBMC Isolation
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Four-hour fasting blood samples were collected on the day of the clinical assessments. Blood samples were collected into 10 mL BD Vacutainer K2-EDTA collection tubes (Becton Dickinson, #367525) for plasma and 8.5 mL BD Vacutainer SSTII advance collection tubes (Becton Dickinson, #367958) for serum, centrifuged immediately (4500 rpm, 15 min at 4°C), pipetted into CryoPure cryogenic tubes (Sarstedt AG & Co, #72.380), and stored at − 80°C. For the isolation of peripheral blood mononuclear cell (PBMC) for immune phenotyping, the SepMate™ PBMC Isolation Tubes (StemCell Technologies, #85460) with Lymphoprep™ (StemCell Technologies, #07861) was used to isolate PBMC from whole blood, according to the manufacturer’s instructions. Purified PBMC were cryopreserved in 10% DMSO (Sigma-Aldrich, #276855) in fetal bovine serum (FBS) (Bovogen, #SFBS-AU).
7
Blood Biomarker Analysis Protocol
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Venous blood was collected using a standard butterfly needle (BD Vacutainer, Becton, Dickson and Company, Franklin Lakes, NJ, USA) or an intravenous catheter (Introcan Safety, Braun Medical Inc., Bethlehem, PA, USA). Blood lipids (highdensity lipoprotein cholesterol (HDL-C), LDL-C, total cholesterol (TC), and TG), hemoglobin A1c, FSH, and estradiol were assessed using standard assays at the University of Colorado-Health/Poudre Valley Hospital Lab (Fort Collins, CO, USA).
Remaining venous blood was processed for serum and plasma separation using VACUETTE® CAT Serum Sep Clot Activator tubes (Greiner Bio-One North America Inc., Monroe, NC, USA) and BD Vacutainer® K2 EDTA collection tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), respectively and stored at -80 °C until analysis. Plasma intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) levels were measured in duplicate using commercially available quantikine ICAM-1 and VCAM-1 enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Bio-Techne brand, Minneapolis, MN, USA). Plasma nitrate/nitrite was measured in duplicate using a commercially available colorimetric assay kit (Cayman Chemical, Ann Arbor, MI, USA).
Remaining venous blood was processed for serum and plasma separation using VACUETTE® CAT Serum Sep Clot Activator tubes (Greiner Bio-One North America Inc., Monroe, NC, USA) and BD Vacutainer® K2 EDTA collection tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), respectively and stored at -80 °C until analysis. Plasma intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) levels were measured in duplicate using commercially available quantikine ICAM-1 and VCAM-1 enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Bio-Techne brand, Minneapolis, MN, USA). Plasma nitrate/nitrite was measured in duplicate using a commercially available colorimetric assay kit (Cayman Chemical, Ann Arbor, MI, USA).
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