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Sensoquest labcycler

Manufactured by Geneflow
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The SensOQuest LabCycler is a thermal cycler designed for DNA amplification and other temperature-dependent biomolecular reactions. It features a precise temperature control system and a user-friendly interface for programming thermal cycling protocols.

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Lab products found in correlation

High capacity rna to cdna kit, by Thermo Fisher Scientific (1 mentions) Steponeplus real time pcr system machine, by Thermo Fisher Scientific (1 mentions) Power sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions)

2 protocols using sensoquest labcycler

1

Thermostability Evaluation of ChTK-F

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The thermostability of ChTK-F was investigated by incubating enzyme samples at a range of temperatures (20–90°C) for one hour using the gradient function in a SensOQuest LabCycler (Geneflow) before samples are cooled to 4°C and assayed for activity using the HPLC method described above.
James P., Isupov M.N., De Rose S.A., Sayer C., Cole I.S, & Littlechild J.A. (2020). A ‘Split-Gene’ Transketolase From the Hyper-Thermophilic Bacterium Carboxydothermus hydrogenoformans: Structure and Biochemical Characterization. Frontiers in Microbiology, 11, 592353.
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2

Efficient RNA Extraction and Quantitative PCR

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RNA was extracted from cells and tissue with a RNeasy mini kit (QIAGEN), with cDNA conversion carried out with a high-capacity RNA-to-cDNA kit (Applied Biosystems) using a SensoQuest Labcycler (Geneflow). Quantitative PCR amplification was performed with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) with 15 ng/well of cDNA, using a StepOnePlus Real-Time PCR System machine (Applied Biosystems). Relative gene expression was calculated with the 2−ΔΔCt method, normalized to the housekeeper gene (SDHA for hearts, UBC for cells). Primer sequences are located in Supplementary Table 1.
Sousa Fialho M.D., Purnama U., Dennis K.M., Montes Aparicio C.N., Castro-Guarda M., Massourides E., Tyler D.J., Carr C.A, & Heather L.C. (2021). Activation of HIF1α Rescues the Hypoxic Response and Reverses Metabolic Dysfunction in the Diabetic Heart. Diabetes, 70(11), 2518-2531.
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