Uas mcherry nls

Manufactured by BD

The UAS-mCherry.NLS is a fluorescent protein-based labeling system for live-cell imaging applications. It utilizes a nuclear localization signal (NLS) to target the mCherry fluorescent protein to the cell nucleus, enabling visualization and tracking of nuclear dynamics. The core function of this product is to provide a fluorescent marker for high-resolution imaging of nuclear structures and processes.

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Lab products found in correlation

Uas lacz, by BD (1 mentions) Uas mcherrycaax, by BD (1 mentions) Uas dicer2, by BD (1 mentions) Uas lifeact gfp, by BD (1 mentions)

2 protocols using uas mcherry nls

Drosophila Genetics and Embryo Staging

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Flies were grown under standard conditions⁴⁵ and crosses were performed at 25 °C or 29 °C (for GAL4/UAS experiments). Staging of embryos was done according to⁴⁶. The following mutations and fly stocks were used in this study: as control or wild-type stocks we employed white¹¹¹⁸ or balanced sibling embryos. We use mef2-GAL4 (BDSC 27390), UAS-mCherry.NLS (BDSC 38425), UAS-lacZ (BDSC 1777), srp³ (BDSC 2485), srp^neo45 (BDSC 59020), srp::GFP (VDRC 318053) and UAS-srpNC^{27 (link)}.

Töpfer U., Bischoff M.C., Bartkuhn M, & Holz A. (2019). Serpent/dGATAb regulates Laminin B1 and Laminin B2 expression during Drosophila embryogenesis. Scientific Reports, 9, 15910.

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Imaging Drosophila Cytoskeleton and Lineage

Cited 1 time

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The following Drosophila stocks were used: R10H12-gal4 (Pfeiffer et al., 2008 (link); BDSC 48278), UAS-mCherryCAAX (BDSC 59021), UAS-mCherryNLS (BDSC 38425), enhancer trap lines sr-gal4^md710 (Usui et al., 2004 (link), BDSC 2663), UAS-Lifeact.GFP (BDSC 35544), UAS-dar1RNAi (BDCS 31987), dar1³⁰¹⁰ (BDSC 65269), UAS-GC3Ai (Schott et al., 2017 (link), BDSC 84346), UAS-serpRNAi (BDSC 63556), UAS-vermRNAi (BDSC 57188), UAS-lolalRNAi (BDSC 35722), UAS-Dicer2 (BDSC 24650), UAS-SrDN (gift from Vorbrüggen and Jäckle, 1997 (link)) and G-TRACE line (Evans et al., 2009 (link), BDSC 28280).

Laurichesse Q., Moucaud B., Laddada L., Renaud Y., Jagla K, & Soler C. (2021). Transcriptomic and Genetic Analyses Identify the Krüppel-Like Factor Dar1 as a New Regulator of Tube-Shaped Long Tendon Development. Frontiers in Cell and Developmental Biology, 9, 747563.

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