Ab24552

Manufactured by Abcam

Sourced in United States

Ab24552 is a laboratory equipment product. It serves a core function related to the product's intended use, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using ab24552

Comprehensive Antibody Collection for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Santa Cruz Biotechnology, Inc. Dallas, Texas, USA: ERα (HC-20) # sc-543, NF-κB p65 (C-20) # sc-372, NF-κB p65 (F-6) # sc-8008, STAT3 (C-20) # sc-482. Abcam, Cambridge, MA, USA: histone H3 (trimethyl K4) # ab8580, GFP antibody # ab290, NCoR # ab24552, KAT13A/SRC1 # ab2859. Thermo Fisher: CBP # PA5-27369. MilliporeSigma: RNA polymerase II (clone 8WG16) # 05-952, normal rabbit IgG # 12-370, normal mouse IgG # 12-371.

Castellaro A.M., Rodriguez-Baili M.C., Di Tada C.E, & Gil G.A. (2019). Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells. Cancers, 11(2), 189.

+ Open protocol

+ Expand

Epigenetic Regulation Immunoprecipitation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunoprecipitation, cells were lysed in RIPA buffer directly followed by sonication. Clarified cell lysates were incubated with the indicated antibodies overnight, and protein A/G beads (Santa Cruz Biotechnology, Inc.) were added for 3–5 h. Beads were washed four times with RIPA buffer. Proteins were eluted in SDS-sample buffer and subjected to western blot analyses. Band intensity was quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA).
The following antibodies were used: LC3B (L7543; Sigma), p62 (M162-3B; MBL International Corporation, Woburn, MA, USA), DNMT1 (ab13537; Abcam), DNMT3A (ab2850; Abcam), DNMT3B (ab16049; Abcam), HDAC1 (sc7872; Santa Cruz Biotechnology Inc.), HDAC2 (sc7899; Santa Cruz Biotechnology Inc.), HDAC3 (sc376957; Santa Cruz Biotechnology Inc.), NCoR (ab24552; Abcam), acetyled Histone H3 (06-599; Sigma-Aldrich), Histone H3 (ab1791; Abcam), Histone H3K9ac (07-352; Sigma-Aldrich), Histone H3K4me3 (39915; Active Motif), Histone H3K27me3 (ab6002; Abcam), MeCP2 (ab2828; Abcam), and β-actin (A5441; Sigma).

Lee M., Nam H.Y., Kang H.B., Lee W.H., Lee G.H., Sung G.J., Han M.W., Cho K.J., Chang E.J., Choi K.C., Kim S.W, & Kim S.Y. (2021). Epigenetic regulation of p62/SQSTM1 overcomes the radioresistance of head and neck cancer cells via autophagy-dependent senescence induction. Cell Death & Disease, 12(3), 250.

+ Open protocol

+ Expand

Analyzing Transcriptional Regulators in Mycobacterial Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-LXRα (ab41902 Abcam), anti-ATF1 (sc270, Santa Cruz), anti-ATF3 (sc188, Santa Cruz), anti-ATF5 (sc377168, Santa Cruz), anti-RXRA (sc553, Santa Cruz), anti-RARA (sc551, Santa Cruz), anti-MEF2A (ab7606. Abcam), anti-MEF2C (ab64644, Abcam), anti-LC3 (NB100-2220, Novus), anti-β-actin (sc-1616, Santa Cruz), anti-H3K27ac (ab4729),anti-NCoR (ab24552), anti-KAT3B/p300 (ab14984), anti-KAT13A/SRC1/NCoA1 (ab84), anti-H3K4me1 (ab8895, abcam), anti-PPARγ (ab41928, Abcam), anti-Mtb-FITC (ab20962, Abcam), anti-rabbit Alexa647 (A-21245, Life Technologies), Prolong Gold with Dapi (Life Technologies), anti-rabbit-Alexa488 (Life Technologies), Bodipy 493 (Life Technologies). TO901317, ATRA and BAX Inhibiting Peptide V5 were bought from Sigma and resupended in DMSO. Nelfinavir (Nel) and Ritonavir (Rit) were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Diseases. Auto Histone ChIP-seq Kit (Protein A) was bought from Diagenode.

Bouttier M., Laperriere D., Memari B., Mangiapane J., Fiore A., Mitchell E., Verway M., Behr M.A., Sladek R., Barreiro L.B., Mader S, & White J.H. (2016). Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Research, 44(22), 10571-10587.

+ Open protocol

+ Expand

Immunological Hybridization Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols

For all the immunological hybridization experiments, the following primary antibodies were used: rabbit polyclonal anti-DLK1 (P10636; Proteintech Group), anti-NCOR1 (ab24552; Abcam), normal rabbit IgG (#2729; Cell Signaling Technology), mouse mono anti-NCOR1 (NBP1-28863; Novus Biologicals). A monoclonal mouse anti-β-actin (sc-7210; Santa Cruz Biotechnology) or anti-GAPDH (sc-137179; Santa Cruz Biotechnology) antibody was used as a marker of equal loading. Goat polyclonal anti-mouse IgG-horseradish peroxidase (HRP) (ab205719; Abcam), anti-mouse IgG-Alexa Fluor® 555 (#4409; Cell Signaling Technology), anti-rabbit IgG-HRP (ab6721; Abcam) anti-rabbit IgG-Alexa Fluor® 488 (#4412; Cell Signaling Technology) were used as secondary antibodies. All of the primary and secondary antibodies used in the present study were diluted in a PBS-0.1% Tween 20 solution containing 5% skim milk or 3% BSA.

Tan J., Zhang S., Li L., Mu J., Wang Z., Zhang L., Jiang M., Li W., Yang X., Liu Y, & Gao Y. (2019). Abnormally localized DLK1 interacts with NCOR1 in non-small cell lung cancer cell nuclear. Bioscience Reports, 39(12), BSR20192362.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!