Melting points were recorded using a Gallenkamp melting point apparatus (Gallenkamp, UK) and open capillaries. Melting points are uncorrected. 1H and 13C NMR spectra were measured utilizing by Bruker Avance (400 MHz for 1H, and 100 MHz for 13C) at Institute of Technology, Karlsruhe University, Karlsruhe, Germany. The 1H and 13C chemical shifts were recorded relative to internal standard TMS. Mass spectrometry was performed using electron impact at 70 eV, (FAB-MS): Finnigan MAT 95 at Institute of Organic Chemistry, Karlsruhe Institute of Technology, Karlsruhe, Germany. Elemental analyses were carried out at Karlsruhe Institute of Technology, Karlsruhe, Germany. Mass spectra were recorded on a Varian MAT 312 instrument in EI mode (70 eV), at Institute of Organic Chemistry, Karlsruhe Institute of Technology, Karlsruhe, Germany. IR spectra were performed using KBr pellets that were run on a FT-IR (Bruker) at Institute of Organic Chemistry, Karlsruhe Institute of Technology, Karlsruhe, Germany. Preparative thin layer chromatography (plc) and glass plates (20 × 48 cm) were covered with a slurry of silica gel (Merck PF254) then air dried and developed using the solvents listed. Zones were detected by quenching of the indicator fluorescence upon exposure to 254 nm UV light.
Pf254
Manufactured by Merck Group
Sourced in Germany
The PF254 is a laboratory equipment product designed for use in scientific research and analysis. It is a high-performance, multi-purpose device that can be utilized in various applications within the scientific field. The core function of the PF254 is to provide reliable and accurate measurements, data processing, and analysis capabilities to support research activities.
Automatically generated - may contain errors
Lab products found in correlation
M 569 sample loader, by Büchi (2 mentions)
U 3200 spectrophotometer, by Shimadzu (2 mentions)
8900 ft ir spectrophotometer, by Shimadzu (2 mentions)
Melting point apparatus, by Gallenkamp (1 mentions)
Avance 500, by Bruker (1 mentions)
Multiskan fc, by Thermo Fisher Scientific (1 mentions)
Mat95xp, by Thermo Fisher Scientific (1 mentions)
M 560, by Büchi (1 mentions)
Bamhi, by Thermo Fisher Scientific (1 mentions)
Chns 932 analyzer, by Leco (1 mentions)
Microflex lt maldi tof mass spectrometer, by Bruker (1 mentions)
100 ftir spectrophotometer, by PerkinElmer (1 mentions)
Hindiii, by Thermo Fisher Scientific (1 mentions)
Mr 400 mhz spectrometer, by Agilent Technologies (1 mentions)
Uv 1800 uv vis spectrometer, by Shimadzu (1 mentions)
Ls 55 fluorimeter, by PerkinElmer (1 mentions)
Ms route, by JEOL (1 mentions)
Avance 3 av 400 mhz, by Bruker (1 mentions)
7 protocols using pf254
1
Characterization of Organic Compounds
2
Spectroscopic Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chromatography was conducted with silica gel 60, mesh size 70–230. Preparative TLC (thin layer chromatography) employed silica gel 60 PF254 (Merck, Darmstadt, Germany). Detection of compounds was performed using UV light at 254 and 266 nm with additional visualization by exposure to iodine vapor and CeSO4 spray. 1H and 13C NMR, COSY (correlated spectroscopy), DEPT (distortionless enhancement by polarization transfer), HMBC, and HSQC (heteronuclear single quantum coherence) spectra were recorded by a Bruker Spectrometer at 600 MHz (Bruker, Billerica, MA, USA), chemical shifts (δ) in ppm, coupling constants (J) in Hz were measured.
3
Spectroscopic Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melting points (m.p.) were recorded on a Buchi M−560 melting point apparatus equipped with a Buchi M−569 sample loader. UV spectra were recorded on a Hitachi U-3200 Spectrophotometer while IR spectra were recorded in KBr with a Shimadzu 8900 FT-IR spectrophotometer. 1H NMR (500 MHz) and 13C NMR (125 MHz) spectra were recorded in deuterated solvents such as CDCl3 and C5D5N on a Bruker Avance-500. Coupling constants, J values, are given in Hertz. DEPT 135°, DEPT 90°, HSQC and HMBC NMR experiments were carried out using the conventional pulse sequences. EIMS and HREIMS were obtained with a JEOL MSRoute-600H and Thermo Finnigan MAT 95XP mass spectrometers respectively. Optical rotations were measured on a JASCO Polarimeter P-2000. Thin-layer chromatography (TLC) was performed on TLC cards pre-coated with silica gel (Merck, PF254, 20x20 cm, 0.25 mm) revealed under UV lamp UVITEC 07-22243 at 254 nm and 365 nm and also with ceric(IV)sulphate solution spray or H2SO4 10% spray reagent. Column chromatography was carried out on silica gel (70–230 mesh, Merck). Optical densities were measured on a Thermo Scientific Multiskan FC, Vantaa, Finland.
4
Purification of Compound 1 via TLC and HPLC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The purity of compound 1 and the degree of its transformations were analyzed by TLC (Thin layer chromatography) (silica gel, 20×20, 0.25 mm thick, PF254, Merck, Germany), while silica gel (70–230 mesh, Merck, Germany) was used for column chromatography. Compounds were finally purified on a recycling HPLC (JAI LC-908W, Japan), equipped with YMC L-80 (4–5 μm, 20−50 mm i.d.). Ceric sulphate reagent was used for visualizing the compounds on TLCs. All solvents used for chromatography were of analytical grade.
5
Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemicals and solvents were obtained from commercial sources (Merck and Sigma) and were used without further purification. DNA (supercoiled pBR322) and restriction enzymes (BamHI and HindIII) were purchased from Thermo-Fischer Scientific. Calf thymus DNA was supplied by Sigma. Measurements of 1H NMR and 13C NMR spectra were recorded in CDCl3 and DMSO-d6 on a Varian MR 400 MHz spectrometer using TMS as an internal standard. A Perkin Elmer 100 FTIR spectrophotometer was used to record the infrared spectra of all compounds (4000–400 cm−1). Mass spectra were obtained using a Bruker Microflex LT MALDI-TOF mass spectrometer. Elemental analyses (C, H, and N) were performed using a Leco 932 CHNS analyzer. The UV-vis. measurements were performed on a Shimadzu UV 1800 UV-vis spectrometer. A Perkin Elmer LS 55 Fluorimeter was used for fluorescence spectra. Microwave irradiated reactions were performed using a CEM MDS-2000, and a household microwave oven. The determination of the melting points was performed using a Büchi B-540 instrument. Analytical TLC was performed on precoated silica gel plates (SiO2, Merck PF254). All aqueous solutions were prepared with purified water or ultrapure water purified by a Millipore Milli-Q Plus water purification device.
6
Thin Layer Chromatography Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Silica gel precoated plates (Merck, PF254; 20 × 20, 0.25 mm) were used for TLC. The crude extract was analyzed by TLC using 1:1 ethyl acetate: methanol as a mobile phase.
7
Spectroscopic Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The melting points of the compounds were recorded on a Buchi M-560 melting point apparatus equipped with a Buchi M-569 sample loader. UV spectra were recorded on a Hitachi U-3200 Spectrophotometer while IR spectra were recorded in KBr with a Shimadzu 8900 FT-IR spectrophotometer. 1 H NMR (400 MHz) and 13 C NMR (100 MHz) spectra were recorded in deuterated solvents (CDCl 3 and Pyridine-d 5 ) on a Bruker Avance III, AV-400 MHz. Coupling constants, J values, are given in Hertz. DEPT 135 o , DEPT 90 o , HSQC, HMBC and 1 H-1 H COSY 2D NMR experiments were carried out using the conventional pulse sequences. EI-MS were obtained with a JEOL MSRoute. Thin-layer chromatography (TLC) was performed on TLC cards pre-coated with silica gel (Merck, PF 254 , 20x20 cm, 0.25mm) revealed under UV lamp UVITEC 07-22243 at 254 nm and 365 nm and also with ceric(IV)sulphate solution spray or H 2 SO 4 10% spray reagent. Column chromatography was carried out on silica gel 60 (0.063-0.200 mm, Merck).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!