Gsdmd antibody

Caspase-1 (Lot: #ab179515), NLRP3 (Lot: #ab263899), and S100 antibodies (Lot: #ab52642) were purchased from Abcam (Cambridge, England, UK). GSDMD antibody (Lot: #E9S1X) and β-actin antibody (Lot: #4967) for western blotting were purchased from Cell Signaling Technology (Massachusetts, USA). The GSDMD (Lot: #20770-1-AP) and βIII-Tubulin (Lot: #66375-1-Ig) primary antibodies for immunofluorescence were purchased from Proteintech (Wuhan, China). The NeuN primary antibody (Lot: #GB11138) was obtained from Servicebio (Wuhan, China). The rat anti-IL-18 antibody (Lot: #CZU0220031) and anti-IL-1β antibody (Lot: #YR0920081) were obtained from R&D Systems (Minneapolis, MN, USA).

Antibodies were as follows: GSDMD antibody (Lot: #E9S1X) was purchased from Cell Signaling Technology (Massachusetts, USA). Caspase-1 (Lot: #ab179515) and S100 antibodies (Lot: #ab52642) were purchased from Abcam (Cambridge, England, UK). NLRP3 (Lot: # T55651) and Rab32 (Lot: # TD9825) was purchased from Abmart (Shanghai, China).
Reagents were as follows: Rat IL-1β (Lot: #E-EL-R0012c) and IL-18 (Lot: #E-EL-R0567c) ELISA Kits were obtained from Elabscience (Shanghai, China). Lipopolysaccharides (LPS, Lot: #L4391-1MG) and adenosine triphosphate disodium (ATP, Lot: #A2383-5G) was purchased from Sigma-Aldrich. We obtained Mitoquinone mesylate (MitoQ, Lot: # HY-100116A) from MedChemExpress (Monmouth Junction, NJ, USA).