Yeast extract peptone dextrose medium

The GenBank accession numbers of Ochrobactrum sp. M231 MPH and OPHC2 are ACC63894 and CAE53631, respectively. Escherichia coli strain Top10 and the plasmid pGEM-T were purchased from Promega Corp. (Madison, WI, USA). E. coli strain BL21(DE3) and the expression plasmid pET-30a(+) were purchased from Novagen (Darmstadt, Germany). P. pastoris strain GS115 and the vector pPIC9 were purchased from Invitrogen (Carlsbad, CA).
E. coli cells were cultured aerobically at 37°C in Luria-Bertani medium. Minimal dextrose (MD) medium, buffered complex glycerol medium (BMGY), yeast extract peptone dextrose medium (YPD), and buffered complex menthol medium (BMMY) were prepared according to the manufacturer’s instructions (Invitrogen).

Escherichia coli JM109, P. pastoris GS115 and the plasmid pPIC9K were purchased from Invitrogen (Carlsbad, CA, USA). Guaiacol, 2,6-dimethoxyphenol (2,6-DMP), and 2,2′-azino-bis(3-ethylbenzothazoline-6-sulfonate) (ABTS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The error-prone PCR kit was acquired from Aviva Systems Biology (Beijing, China). All other chemicals and reagents were of analytical grade. Yeast Extract Peptone Dextrose Medium (YPD), Minimal Dextrose Medium (MD), Buffered Glycerol-Complex Medium (BMGY) and Buffered Minimal Methanol Medium (BMM) were prepared following the instructions from Invitrogen.