Goat anti human igg cy3

Manufactured by Jackson ImmunoResearch

Sourced in United States

Goat anti-human IgG-Cy3 is a secondary antibody conjugated with the fluorescent dye Cy3. It is designed to detect and visualize human immunoglobulin G (IgG) in various immunoassays and applications.

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Lab products found in correlation

Facs lsr 2 instrument, by BD (1 mentions) Cover slip, by Marienfeld (1 mentions) Immersol 518f, by Zeiss (1 mentions) Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Lsm 880 confocal laser scanning microscope, by Zeiss (1 mentions)

Spelling variants of this product

Cy3-conjugated goat anti-human IgG (14 citations) Cy3-goat-anti-human-IgG (H+L), (4 citations) Cy3-anti-human IgG (3 citations) Cy3 anti-goat (3 citations)

3 protocols using goat anti human igg cy3

Quantitative Flow Cytometry of Sporozoite Antibodies

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10⁵Pb-PfCSP salivary gland sporozoites were incubated in siliconized tubes with 100 µg/ml or 1 µg/ml of mAbs in a total volume of 60 µl PBS with 1% (vol/vol) FCS for 30 min on ice. The sporozoites were washed with 1.5 ml of 1% (vol/vol) FCS in PBS by centrifuging at 9,300 g for 4 min at 4°C. The sporozoite pellet was resuspended in 100 µl of 1% (vol/vol) FCS in PBS containing goat anti–human IgG-Cy3 (1:1,000; Jackson ImmunoResearch) and incubated for 30 min on ice. The sporozoite suspension was washed once more, and the pellet was resuspended in 200 µl of 1% (vol/vol) FCS in PBS. Antibody-stained sporozoites were detected by intrinsic GFP expression and Cy3 staining in a FACS LSR II instrument (Becton Dickinson). Data were analyzed using FlowJo V.10.0.8 (Tree Star).

Scally S.W., Murugan R., Bosch A., Triller G., Costa G., Mordmüller B., Kremsner P.G., Sim B.K., Hoffman S.L., Levashina E.A., Wardemann H, & Julien J.P. (2018). Rare PfCSP C-terminal antibodies induced by live sporozoite vaccination are ineffective against malaria infection. The Journal of Experimental Medicine, 215(1), 63-75.

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Serum IgG and IgM Immunofluorescence Assay

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The slides were allowed to reach room temperature and serum specimens were fully thawed before testing. Serum samples were diluted 1:20 in freshly filtered 1% casein and then centrifuged (15 min, 13,300 RCF), to remove aggregates. Diluted serum (135 μl) was dispensed using pipette at the center of the slide, covered with a cover slip (24×60 mm, Marienfeld) and then incubated in a humidity chamber (1h, 37 °C). Each slide was then dipped in PBS, to remove the cover slips, and then sequentially washed in PBS, PBS-T, and PBS for ten minutes each, before being dipped in reverse osmosis-grade water. The slides were dried in a Nuve centrifuge NF800 (10 min, 2180 rpm) before being incubated with goat anti-human IgG-Cy3 (Jackson ImmunoResearch Laboratory) and goat anti-human IgM-Dy5 (Jackson ImmunoResearch Laboratory) diluted 1:500 in 1% casein in PBS. All washing solutions were at room temperature. The slides after testing were stored at 4°C, under vacuum, until scanning.

Putterman C., Wu A., Reiner-Benaim A., Batty DS J.r., Sanz I., Oates J., Jakobi K., Petri M., Safer P., Gerwien R., Sorek R., Blumenstein Y, & Cohen I.R. (2015). SLE-key® Rule-Out Serologic Test for Excluding the Diagnosis of Systemic Lupus Erythematosus: Developing the ImmunArray iCHIP®. Journal of immunological methods, 429, 1-6.

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Immunostaining of Human Liver Cryosections

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Immunostaining of liver cryosections was carried out as previously described.¹³ (link) Briefly, methanol-fixed cryosections of non-cholestatic human liver were immunostained with human serum samples at 1:100 for anticanalicular reactivity and a murine monoclonal antibody against multidrug resistance-associated protein 2 (MRP2; M2I-4; Alexis, Grünberg, Germany) as a canalicular marker (1:25). Goat antihuman-IgG-Cy3 (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) and goat antimouse-IgG-Alexa Fluor 488 (Invitrogen, Karlsruhe, Germany) served as secondary antibodies (both at 1:500). All images were acquired via the ZEN software (black edition, Ver.2.3 SP1) on an LSM 880 confocal laser scanning microscope (Zeiss, Jena, Germany) using Immersol 518 F (Zeiss) oil on a Zeiss Plan-APOCHROMAT 63 × Oil/DIC objective at room temperature.

Stindt J., Dröge C., Lainka E., Kathemann S., Pfister E.D., Baumann U., Stalke A., Grabhorn E., Shagrani M.A., Mozer-Glassberg Y., Hartley J., Wammers M., Klindt C., Philippski P., Liebe R., Herebian D., Mayatepek E., Berg T., Schmidt-Choudhury A., Wiek C., Hanenberg H., Luedde T, & Keitel V. (2023). Cell-based BSEP trans-inhibition: A novel, non-invasive test for diagnosis of antibody-induced BSEP deficiency. JHEP Reports, 5(7), 100690.

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