Mouse tgf β1 enzyme linked immunosorbent assay elisa kit

Baicalin was obtained from Sigma (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide, then diluted by sterile saline. Bleomycin hydrochloride was obtained from Nippon Kayaku (Co, Tokyo, Japan). Hydroxyproline (HYP) assay kits were obtained from Nanjing Jiancheng Biochemical Institute (Nanjing, China). Mouse TGF-β1 enzyme linked immunosorbent assay (ELISA) kit was purchased from R&D (Minneapolis, MN, USA). The rabbit antibodies against ERK1/2, phospho-ERK1/2, and GAPDH were purchased from Cell Signaling Technology (Beverly, MA, USA). The mouse antibodies against TGF-β1 and A2aR were purchased from Abcam (Cambridge, UK). Peroxidase conjugated goat anti-rabbit immunoglobulin G (IgG) and Peroxidase conjugated goat anti-mouse immunoglobulin G (IgG) was purchased by Boster (Wuhan, China). DAB kit and Polink-2 plus Polymer HRP Detection System were provided by ZSGB BIO (Beijing, China). SuperSignal (R) West Femto Maximum Sensitivity Substrate and BCA Protein Assay kit were provided by Thermo Fisher Scientific (Waltham, MA, USA).

Serum levels of the inflammatory cytokines murine IL-1β, IL-6, interferon (IFN)-γ, TNF-α, and IL-10 were determined using the Luminex multiplex cytokine assay (Luminex 200 system, Millipore, Billerica, MA, USA) according to the manufacturer’s recommendations. The level of TGFβ1 was measured using a commercially available mouse TGFβ1 enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems).