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Dnase 1 collagenase

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DNAse I/Collagenase is a laboratory reagent used in biological research. It is a mixture of two enzymes, DNAse I and collagenase, which function to degrade DNA and collagen, respectively. This product is commonly used in cell isolation and tissue dissociation protocols.

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Lab products found in correlation

Countbright, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

DNase I (4163 citations) Collagenase (2150 citations) DNase (1597 citations) Collagenase I (700 citations) Collagenase/DNAse (8 citations) Collagenases I (2 citations) DNase I (DNase; (2 citations) Collagenase type IV and DNase I (2 citations) Collagenase type IV/DNase I (2 citations)

2 protocols using dnase 1 collagenase

1

Quantifying Osx/GFP+ Cells in T-ALL

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T-ALL engraftment and infiltration was confirmed via peripheral blood sampling and/or tibia puncture. Once mice presented with signs of ill health (as described earlier), mice were euthanized and bones digested with a DNAse I/Collagenase (Sigma) solution. The total number of Osx/GFP+ cells was assessed by flow cytometry analysis using counting beads (CountBright, Life Technologies).
Hawkins E.D., Duarte D., Akinduro O., Khorshed R.A., Passaro D., Nowicka M., Straszkowski L., Scott M.K., Rothery S., Ruivo N., Foster K., Waibel M., Johnstone R.W., Harrison S.J., Westerman D.A., Quach H., Gribben J., Robinson M.D., Purton L.E., Bonnet D, & Lo Celso C. (2016). T cell acute leukaemia exhibits dynamic interactions with bone marrow microenvironments. Nature, 538(7626), 518-522.
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2

Quantifying Osx/GFP+ Cells in T-ALL

Check if the same lab product or an alternative is used in the 5 most similar protocols
T-ALL engraftment and infiltration was confirmed via peripheral blood sampling and/or tibia puncture. Once mice presented with signs of ill health (as described earlier), mice were euthanized and bones digested with a DNAse I/Collagenase (Sigma) solution. The total number of Osx/GFP+ cells was assessed by flow cytometry analysis using counting beads (CountBright, Life Technologies).
Hawkins E.D., Duarte D., Akinduro O., Khorshed R.A., Passaro D., Nowicka M., Straszkowski L., Scott M.K., Rothery S., Ruivo N., Foster K., Waibel M., Johnstone R.W., Harrison S.J., Westerman D.A., Quach H., Gribben J., Robinson M.D., Purton L.E., Bonnet D, & Lo Celso C. (2016). T cell acute leukaemia exhibits dynamic interactions with bone marrow microenvironments. Nature, 538(7626), 518-522.
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