Ix81 spin disk confocal microscope

Manufactured by Olympus

Sourced in Japan

The IX81 spin disk confocal microscope is a high-performance imaging system designed for advanced fluorescence microscopy. It utilizes a spinning disk technology to provide fast, high-resolution imaging with minimal phototoxicity. The system is capable of acquiring images at high speeds and with excellent optical sectioning capabilities, making it suitable for a wide range of live-cell and fixed-sample applications.

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Lab products found in correlation

Fluo 4 am solution, by Thermo Fisher Scientific (2 mentions)

2 protocols using ix81 spin disk confocal microscope

Quantifying Calcium Dynamics in Cholangiocytes

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For calcium dye loading, cholangiocytes were incubated with 5 μM of Fluo4‐AM solution (Thermo Fisher Scientific) and placed on a rocker for 2 hours at 37°C. Excess dye was washed away with Hanks' balanced salt solution (HBSS) before flow experiments. The bile duct‐on‐a‐chip was connected to a syringe pump to apply shear flow with HBSS at 3.9 dyne/cm². Time‐lapse images were acquired at 1‐second intervals for 3 minutes using an Olympus IX81 spin disk confocal microscope (Olympus, Tokyo, Japan).

Du Y., Khandekar G., Llewellyn J., Polacheck W., Chen C.S, & Wells R.G. (2019). A Bile Duct‐on‐a‐Chip With Organ‐Level Functions. Hepatology (Baltimore, Md.), 71(4), 1350-1363.

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Calcium Dye Imaging of Cholangiocytes

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For calcium dye loading, cholangiocytes were incubated with 5 μM Fluo4-AM solution (Thermo Scientific) and placed on a rocker for 2 h at 37°C. Excess dye was washed away with HBSS before flow experiments. The bile duct-on-a-chip was connected to a syringe pump to apply shear flow with HBSS at 3.9 dyne/cm². Time-lapse images were acquired at 1 sec intervals for 3 min using an Olympus IX81 spin disk confocal microscope (Olympus, Japan).

Du Y., Khandekar G., Llewellyn J., Polacheck W., Chen C.S, & Wells R.G. (2019). A Bile Duct‐on‐a‐Chip With Organ‐Level Functions. Hepatology (Baltimore, Md.), 71(4), 1350-1363.

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