Gst resin

Anti-6His-XL665 Cryptate antibodies and anti-GST-Eu Cryptate antibodies, white 384-shallow well microplate were purchased from PerkinElmer. 96-well Black microplates were purchased from Greiner Bio-One. Streptavidin (SA) biosensors were purchased from Sartorius ForteBio. EZ-Link™ Sulfo-NHS-LC-LC-Biotin was purchased from Thermo Fisher Scientific. BI224436 was purchased from MedChem Express (Shanghai, China). Compounds were obtained from National Compound Resource Centre (Shanghai, China) and stock solutions (10 mM) were stored protected from light at −80 °C. Ni-NTA resin and GST resin were purchased from Smart-Lifesciences (Changzhou, China). All general biochemical reagents were obtained from AMRESCO (Solon, USA).

All general biochemical reagents were obtained from AMRESCO (Solon, USA). Ni-NTA resin and GST resin were purchased from Smart-Lifesciences (Changzhou, China). Ni-NTA Magnetic Agarose Beads were purchased from BEAVER Nano-technologies (Suzhou, China). 96-well nonbinding surface (NBS) microplates were purchased from Corning (New York, USA). Rabbit anti-mouse IgG alkaline phosphatase conjugated antibody and mouse anti-GST antibody were purchased from Sigma (St. Louis, USA). INH5 was synthesized by GL Biochem (Shanghai, China).

All general biochemical reagents were obtained from AMRESCO (Solon, USA). Ni-NTA resin and GST resin were purchased from Smart-Lifesciences (Changzhou, China). Anti-6His-XL665- and anti-GST-Eu Cryptate antibodies were purchased from Cisbio. 96-well Black microplates were purchased from Greiner Bio-One. White 384-shallow well microplate was purchased from PerkinElmer. Streptavidin (SA) biosensors were purchased from Pall ForteBio. EZ-Link™ Sulfo-NHS-LC-LC-Biotin were purchased from Thermo Fisher Scientific. Ebselen was purchased from MedChem Express (Shanghai, China). Three protein kinase inhibitor libraries (Syn kinase inhibitor library, protein kinase inhibitor library and kinase inhibitor library) and REDOX library were obtained from National Compound Resource Center (Shanghai, China).