Complex 2 enzyme activity assay kit

Manufactured by Abcam

Sourced in United States

The Complex II Enzyme Activity Assay Kit is a tool used to measure the activity of Complex II, also known as succinate dehydrogenase, a key enzyme in the mitochondrial electron transport chain. The kit provides the necessary reagents and protocols to quantify the enzymatic activity of Complex II in a simple and reliable manner.

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Lab products found in correlation

Complex 4 enzyme activity assay kit, by Abcam (2 mentions) Mitochondrial complex 3 activity assay kit, by Abcam (2 mentions) Complex 1 enzyme activity assay kit, by Abcam (2 mentions) Pherastar fs, by BMG Labtech (1 mentions) Mitochondria isolation kit for cultured cells, by Abcam (1 mentions) Mitochondria isolation kit for tissue, by Abcam (1 mentions)

3 protocols using complex 2 enzyme activity assay kit

Measuring Complex II Activity

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Frozen tissue was homogenized in a PBS buffer supplemented with protease inhibitors and prepared for complex II activity measurements using a Complex II Enzyme activity assay kit (Abcam, ab109908). Colorimetric measurements were achieved using PheraStar FS (BMG Labtech, Aylesbury, UK).

Jespersen N.R., Hjortbak M.V., Lassen T.R., Støttrup N.B., Johnsen J., Tonnesen P.T., Larsen S., Kimose H.H, & Bøtker H.E. (2020). Cardioprotective effect of succinate dehydrogenase inhibition in rat hearts and human myocardium with and without diabetes mellitus. Scientific Reports, 10, 10344.

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Mitochondrial Enzyme Activity Assays

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Herein, mitochondria in cell samples were isolated using Mitochondria Isolation Kit for Cultured Cells (ab110170, Abcam, USA). Then, the activity of Complex I to IV was evaluated using Complex I Enzyme Activity Assay Kit (ab109721, Abcam, USA), Complex II Enzyme Activity Assay Kit (ab109908, Abcam, USA), Mitochondrial Complex III Activity Assay Kit (K520-100, Biovision, USA), and Complex IV Enzyme Activity Assay Kit (ab109909, Abcam, USA) according to the manufacturer’s instructions respectively.

Feng Y., Xu J., Shi M., Liu R., Zhao L., Chen X., Li M., Zhao Y., Chen J., Du W, & Liu P. (2022). COX7A1 enhances the sensitivity of human NSCLC cells to cystine deprivation-induced ferroptosis via regulating mitochondrial metabolism. Cell Death & Disease, 13(11), 988.

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Mitochondrial ETC Complex Activity Assay

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In this study, mitochondria was first isolated from tissue samples with Mitochondria Isolation Kit for Tissue (ab110169, Abcam). Then, the activity of Complex I–IV in mitochondrial ETC was measured using Complex I Enzyme Activity Assay Kit (ab109721, Abcam), Complex II Enzyme Activity Assay Kit (ab109908, Abcam), Mitochondrial Complex III Activity Assay Kit (K520-100, Biovision), and Complex IV Enzyme Activity Assay Kit (ab109909, Abcam) according to the manufacturer’s instructions respectively.

Liu P., Yuan J., Feng Y., Chen X., Wang G, & Zhao L. (2021). Ferroptosis contributes to isoflurane-induced neurotoxicity and learning and memory impairment. Cell Death Discovery, 7, 72.

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