Small interfering rnas sirnas

Mouse monoclonal antibodies to N-cadherin (D-4), vimentin (V9), Snail (G-7), and the large extracellular loop of CD82 (G-2) and rabbit polyclonal antibodies to Slug (H-140), Twist (H-81), FAK (A-17), TM4SF2 (Y-19), phospho-ILK^(T173), and phospho-FAK^(Y925), and the C-terminus of CD82 (C-16) and goat polyclonal antibodies to integrin α3 (I-19), α5 (P-19), and α6 (N-19) subunits and an inhibitor to FAK (FAK inhibitor 14) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Monoclonal Antibodies to integrin β₁ subunit (P5D2) and α-SMA (E184) and an inhibitor to ILK (Cpd22) were purchased from Millipore (Billerica, MA). Monoclonal antibodies to E-cadherin (36/E-cadherin) and fibronectin (C6F10) were obtained from BD Biosciences (San Diego, CA). Antibodies to phospho-Src^(Y416) and ILK were purchased from Cell Signaling (Beverly, MA) and Abcam (Cambridge, UK), respectively. Alexa fluor-conjugated goat anti-mouse (Alexa488) and goat anti-rabbit (Alexa555) secondary antibodies were from Life Technologies (Grand Island, NY). The small interfering RNAs (siRNAs) targeting integrin α₃, α₅, or α₆ subunit were obtained from Bioneer (Daejeon, Korea) and sequences are listed in the supplementary Table S2. All other reagents were from Sigma-Aldrich (St. Louis, Mo) unless indicated otherwise.

The BJAB cell line was kindly provided by Dr. H. Y. Yoo (Sungkyunkwan University, Seoul, Korea). The Raji cell line was kindly provided by Dr. D. H. Nam (Samsung Medical Center, Seoul, Korea). The Riva cell line was purchased from Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zilkulturen GmbH (Braunschweig, Germany). BJAB, Riva, and Raji cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS, penicillin, and streptomycin (Gibco-BRL, Grand Island, NY, USA). The cells were incubated in a humidified 5% CO₂ atmosphere. All cell lines were tested for mycoplasma and characterized by STR profiling as indicated in the DSMZ online. To establish LMP1-expressing stable cell lines, BJAB or Riva cells were transfected with EGFP-N1 control vector or EGFP-N1 + LMP1 vector via electroporation (Lonza, Cologne, Germany) and selected for 1 month with 1 mg/ml G418 (Sigma-Aldrich, Poole, UK). Small interfering RNAs (siRNAs) were purchased from Bioneer (Daejeon, South Korea). Cells were transiently transfected using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA). The CHAC2 expression and pCMV6-entry control vector were purchased from Origene (Rockville, MD, USA) and transfected into cells by electroporation. Doxorubicin and TCP were purchased from Selleckchem (Houston, TX, USA).

UCB-MSCs were acquired from Kang Stem Biotech (Seoul, Korea). Fetal bovine serum (FBS) and antibiotics were purchased from Hyclone (Logan, UT, USA) and Gibco (Grand Island, NY, USA), respectively. 4-P-PDOT (#SML1189), cholesterol (#C4951), DIDS (#D3514), melatonin (#M5250), and Ver155008 (#SML0271) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mithramycin A (#1489) was purchased from Tocris (Minneapolis, MN, USA). Antibodies against ABCA1 (#ab18180), α-SMA (#ab5694), and Sp1 (#ab227383) were purchased from Abcam (Cambridge, England) and against HSF1 (#H00003297-A01) were purchased from Abnova (Taipei, Taiwan). Antibodies against NRF1 (#8052S) and cleaved-caspase 3 (#9661) were purchased from Cell Signaling Technology (Beverly, MA, USA), and those against BiP (#MA5-27686) and phospho-Sp1 (Thr453, #PA5-38333) were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against MT1 (#NBP1-71113) were purchased from Novus Biologicals (Littleton, CO, USA). Antibodies against β-actin (#sc-47778), caspase 9 (#sc-8355), lamin A/C (#sc-20681), and MT2 (#sc-28453) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). All primers for mRNA and miRNA were purchased from Cosmogenetech (Seoul, Korea). Small interfering RNAs (siRNAs) were purchased from Bioneer (Deajeon, Korea).