Dota bn

Manufactured by Macrocyclics

DOTA-Bn is a chemical compound used in various laboratory applications. It serves as a chelating agent, capable of forming stable complexes with metal ions. The core function of DOTA-Bn is to facilitate the binding and detection of specific metal ions in analytical procedures.

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Lab products found in correlation

177lucl3, by PerkinElmer (1 mentions)

Close spelling variants of this product

P-SCN-Bn-DOTA (DOTA) P-SCN-Bn-DOTA S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (p-SCN-Bn-DOTA) 2-(p-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-N,N′,N″,N‴-tetraacetic acid (p-SCN-Bn-DOTA)

2 protocols using dota bn

Labeling of hu3F8-C825 with Radioactive Iodine and Lutetium-177

Cited 1 time

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¹²⁴I was either provided in-house by the Memorial Sloan Kettering Radiochemistry & Molecular Imaging Probes Core Facility or purchased commercially (IBA Molecular). Stocks of hu3F8-C825 were labeled with radioactive iodine using pre-coated IODOGEN tubes (Pierce) according to standard protocols (15 , 16 (link)) to final specific activities of 121-152 MBq/mg. The tracer immunoreactivities were evaluated using cell-binding assays (17 (link)) and were 78.8 ± 0.1% (n = 3) for GD2(+)-NB IMR-32 and 4.9 ± 0.4% (n = 3) for GD2(-) SK-N-SH. The chelate p-aminobenzyl-DOTA (DOTA-Bn; Macrocyclics) was radiolabeled with ¹⁷⁷LuCl₃ (specific activity: 170 MBq/nmol; Perkin Elmer) at a ratio of 37 MBq to 1.1 μmol DOTA-Bn using previously described methods (9 (link)), but without HPLC purification (C825 does not recognize metal-free DOTA-Bn).

Cheal S.M., Xu H., Guo H.F., Zanzonico P.B., Larson S.M, & Cheung N.K. (2014). Preclinical evaluation of multistep targeting of diasialoganglioside GD2 using a IgG-scFv bispecific antibody with high affinity for GD2 and DOTA metal complex. Molecular cancer therapeutics, 13(7), 1803-1812.

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Bispecific Antibody and Chelator Preparation

Cited 1 time

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The BsAb and CA were prepared according to reported methods (19 (link),21 (link)). DOTA-Bn was obtained commercially (Macrocyclics, Plano, TX) and used without any additional purification.

Chandler C.S., Bell M.M., Chung S.K., Veach D.R., Fung E.K., Punzalan B., Vargas D.B., Patel M., Xu H., Guo H.F., Santich B.H., Zanzonico P.B., Monette S., Nash G.M., Cercek A., Jungbluth A., Pandit-Taskar N., Cheung N.K., Larson S.M, & Cheal S.M. (2021). Intraperitoneal pretargeted radioimmunotherapy for colorectal peritoneal carcinomatosis. Molecular cancer therapeutics, 21(1), 125-137.

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