Imobilon psq membrane

Manufactured by Merck Group

Immobilon-PSQ membrane is a polyvinylidene fluoride (PVDF) membrane designed for protein transfer and immobilization in Western blotting applications. It offers high protein-binding capacity and a consistent pore size distribution for efficient protein transfer and retention.

Automatically generated - may contain errors

Lab products found in correlation

Anti flag m2 antibody, by Merck Group (2 mentions) Western lightning plus ecl, by PerkinElmer (2 mentions) Alliance 4, by Uvitec (2 mentions) Anti myc antibody, by Santa Cruz Biotechnology (1 mentions) Dynabeads, by Merck Group (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions)

2 protocols using imobilon psq membrane

Western Blot Analysis of Transfected Clones

Check if the same lab product or an alternative is used in the 5 most similar protocols

For detection of stably transfected MDA-MB-231T clones 10⁶ cells of each clone was collected, washed in PBS and sonicated.
The samples (10 μg) were loaded on SDS-PAGE and electrotransfered to an Imobilon-PSQ membrane (Milipore). For detection of FLAG/NDK and FLAG/H1 the anti-FLAG M2 antibody (Sigma) was used while MYC/H2 was detected using anti-MYC antibody (Santa Cruz Biotechnology). Anti-α-tubulin antibody (Calbiochem) was used as loading control. After application of appropriate secondary antibodies the protein bands were visualized using Western Lightning Plus-ECL (PerkinElmer, Inc.). The image was acquired by Alliance 4.7 (Uvitec) and assembled in Adobe Photoshop.

Fancsalszky L., Monostori E., Farkas Z., Pourkarimi E., Masoudi N., Hargitai B., Bosnar M.H., Deželjin M., Zsákai A., Vellai T., Mehta A, & Takács-Vellai K. (2014). NDK-1, the Homolog of NM23-H1/H2 Regulates Cell Migration and Apoptotic Engulfment in C. elegans. PLoS ONE, 9(3), e92687.

+ Open protocol

+ Expand

Detection of Nme1 Protein Complex

Check if the same lab product or an alternative is used in the 5 most similar protocols

For detection of stably transfected MDA-MB-231T (control), two selected clones expressing the Capsospora variant of the NmeGp1 protein (Co2 and Co9) were seeded in 100 mm Petri dishes, washed two times in PBS, collected in lysis buffer (20 mM Tris, pH 7.6-8, 150 mM NaCl, 1% Triton, and 1 mM EDTA) supplemented with protease inhibitors and degraded by sonication. The immunoprecipitation was done with either anti-Nme1 antibody (Calbiochem) using Dynabeads, or with anti-FLAG M2 affinity gel (Sigma) according to the manufacturer's instructions. Immunoprecipitation with IgG (Sigma) was used as a negative control.
The samples were loaded on SDS-PAGE and electrotransfered to an Imobilon-PSQ membrane (Milipore). The membranes were incubated with anti-FLAG M2 antibody (Sigma) of affinity-purified polyclonal anti-Nme1 antibody for detection of complex formation. Protein bands were visualized using Western Lightning Plus-ECL (PerkinElmer). The images were acquired by Alliance 4.7 (Uvitec) and assembled in Adobe Photoshop.

Ćetković H., Bosnar M.H., Perina D., Mikoč A., Deželjin M., Belužić R., Bilandžija H., Ruiz-Trillo I, & Harcet M. (2018). Characterization of a group I Nme protein of Capsaspora owczarzaki-a close unicellular relative of animals. Laboratory investigation; a journal of technical methods and pathology, 98(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!