H. cordata was purchased from Wuxi Xiaogu e-commerce Co., Ltd. (Chongqing, China). The materials were thoroughly washed with tap water, air-dried at room temperature, and then finely powdered with a pulverizer through 30 mesh sieves. d -glucose, l -rhamnose, d -xylose, d -mannose, d -glucuronic acid, d -galacturonic acid, and Sephadex G-200 were purchased from Sigma Chemical Co., Ltd. (St. Louis, MO, USA). Dextran (T-10, T-40, T-70, T-500, and T-2000) was obtained from Solarbio Company (Beijing, China). All other chemicals used in this study were of analytic grade.
T 500
Manufactured by Solarbio
Sourced in China
The T-500 is a laboratory centrifuge designed for general-purpose applications. It has a maximum speed of 5,000 rpm and a maximum relative centrifugal force (RCF) of 3,000 x g. The T-500 can accommodate a variety of rotor sizes and types, allowing for the separation and extraction of samples in various laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
T 2000, by Solarbio (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
D glucose, by Merck Group (1 mentions)
D galacturonic acid, by Merck Group (1 mentions)
L rhamnose, by Merck Group (1 mentions)
D mannose, by Merck Group (1 mentions)
D xylose, by Merck Group (1 mentions)
D glucuronic acid, by Merck Group (1 mentions)
Sephadex g 200, by Merck Group (1 mentions)
Ascorbic acid, by Sinopharm (1 mentions)
Malonaldehyde, by Nanjing Jiancheng (1 mentions)
Fitc dextran, by Merck Group (1 mentions)
Anti cd11c pe, by BD (1 mentions)
Deae 52 cellulose, by GE Healthcare (1 mentions)
Phosphate buffered solution, by Thermo Fisher Scientific (1 mentions)
Granulocyte macrophage colony stimulating factor gm csf, by Thermo Fisher Scientific (1 mentions)
Anti mhcii pe, by BD (1 mentions)
Anti mhc 1 fitc, by BD (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
D fructose, by Solarbio (1 mentions)
5 protocols using t 500
1
Extraction and Purification of H. cordata
2
Guava Leaves for Antioxidant and Metabolic Evaluation
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Guava leaves were obtained from Jiangmen Nanyue Guava farmer cooperatives (Guangdong, China). Leaves were dried at 60 °C, then pulverized and sieved (40 mesh) for the experiments.
1,1-Diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Macklin Biochemical Co., Ltd. ABTS+ and Trolox were obtained from Aladdin Industrial Corporation (Shanghai, China). Ascorbic acid was purchased from Sinopharm chemical reagent Co., Ltd. (Shanghai, China). Dextran T-2000, T-500, T-70, T-40, and T-10 were purchased from Solarbio (Beijing, China). Acorbose was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Streptozocin was obtained from MP Biomedicals (Santa Ana, CA, USA). Assay kits for total cholesterol (TC), total triglycerides (TG), glycated serum protein (GSP), creatinine (CRE), total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and malonaldehyde (MDA) were purchased from Nanjing jiancheng Bioengineering Institute (Nanjing, China). The high fat diet consisted of 10% lard, 20% sucrose, 10% yolk powder, 0.5% sodium cholate, and 59.5% conventional feed, which was purchased from Jiangsu synergetic pharmaceutical bioengineering Co., Ltd. (Nanjing, China).
1,1-Diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Macklin Biochemical Co., Ltd. ABTS+ and Trolox were obtained from Aladdin Industrial Corporation (Shanghai, China). Ascorbic acid was purchased from Sinopharm chemical reagent Co., Ltd. (Shanghai, China). Dextran T-2000, T-500, T-70, T-40, and T-10 were purchased from Solarbio (Beijing, China). Acorbose was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Streptozocin was obtained from MP Biomedicals (Santa Ana, CA, USA). Assay kits for total cholesterol (TC), total triglycerides (TG), glycated serum protein (GSP), creatinine (CRE), total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and malonaldehyde (MDA) were purchased from Nanjing jiancheng Bioengineering Institute (Nanjing, China). The high fat diet consisted of 10% lard, 20% sucrose, 10% yolk powder, 0.5% sodium cholate, and 59.5% conventional feed, which was purchased from Jiangsu synergetic pharmaceutical bioengineering Co., Ltd. (Nanjing, China).
3
Characterization of Dendritic Cell Activation
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DEAE-52 cellulose was obtained from GE Healthcare (Uppsala, Sweden). The monosaccharides, comprising ribose (Rib), glucuronic acid (GlcA), glucose (Glc), galactose (Gla), mannose (Man), arabinose (Ara), rhamnose (Rha) and xylose (Xyl), lipopolysaccharide (LPS), active carbon, trifluoroacetic acids (TFA), and FITC-Dextran, were purchased from Sigma-Aldrich (St Louis, MO, USA). T-series dextrans (T-10, T-40, T-70, T-500 and Blue dextran) were purchased from Solarbio (Beijing, China). Fetal bovine serum (FBS) and Penicillin-streptomycin were purchased from MRC (Changzhou, China). Medium RPMI-1640 and phosphate-buffered solution (PBS) were purchased from Gibco (Grand Island, NY, USA). Granulocyte-macrophage colony-stimulating factor (GM-CSF) was purchased from PeproTech (Rocky Hill, NJ, USA). The other chemical reagents were purchased from Tianjin Fuchen (Tianjin, China).
The antibodies for flow cytometry comprising anti-CD40-APC, anti-CD86-APC, anti-CD11c-FITC, and ELISA kits including tumor necrosis factor-α (TNF-α), IL-1β, and IL-12p40 were purchased from Elabscience (Wuhan, China). Anti-MHC-I-FITC, anti-MHC-II-PE, and anti-CD11c-PE were bought from BD Biosciences (San Diego, CA, USA).
The antibodies for flow cytometry comprising anti-CD40-APC, anti-CD86-APC, anti-CD11c-FITC, and ELISA kits including tumor necrosis factor-α (TNF-α), IL-1β, and IL-12p40 were purchased from Elabscience (Wuhan, China). Anti-MHC-I-FITC, anti-MHC-II-PE, and anti-CD11c-PE were bought from BD Biosciences (San Diego, CA, USA).
4
Purification and Characterization of Marine Microalgal Polysaccharides
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Crypthecodinium cohnii was supply by Third Insititude Oceanography, State Oceanic Administration. l -rhamnose, d -glucuronic, d -arabinose, dxylose, d -fructose, d -galactose, and d -mannose were purchased from Solarbio Co. (Beijing, China), while HiTrap Capto Q (16/25) and Superose 6B (10/300) were purchased from GE Co. (Fairfield, CT, USA). T-series dextrans (T-10, T-40, T-70, T-500, T-2000) as standard samples with different molecular weights were purchased from Solarbio Co. (Beijing, China). Methyl iodide (CH3I) was purchased from Sigma Co., Ltd. (Shanghai, China). Trifluoroacetic acid (TFA) and sodium periodate (NaIO4) were purchased from BLOT Co., Ltd. (Tianjin, China). 3-(4,5-Dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide, dimethylsulphoxide (DMSO) was purchased from Sigma Co., Ltd. (Shanghai, China). RPMI-1640 medium and fetal bovine serum (FBS) was purchased from Gibco Invitrogen Co. (New York, NY, USA). Primary antibodies were purchased from CST Co. (Cell Signaling Technology, Danvers, MA, USA), secondary antibodies (HRP-IgG) were purchased from Beyotime Co. (Zhengzhou, China). NC (nitrocellulose) membrane for transfering was purchased from PALL Co. (New York, NY, USA). Other chemicals and reagents were of analytical grade.
5
Characterization of Shanxi Cortex Periplocae
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Cortex Periplocae produced in Shanxi Province of China was purchased from Zhang Zhongjing Pharmaceutical Ltd. (Zhengzhou, China) DEAE-52; Sephadex G-100; DPPH; ABTS; TCA; Vc (>99.7%); K3Fe(CN)6; papain; TFA; TPTZ; monosaccharide standards (Rha, Ara, Man, Glc, Gal, Fru, and GlcA); and Dextran Mw standards (T-10, T-40, T-100, T-500, and T-1000) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).
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