Human NSCLC cell lines such as A549 (KRAS.G12S), NCI-H1299 [H1299] (NRAS Q61K), NCI-H23 [H23] (KRAS G12C), NCI-H460 [H460] (KRAS Q61H), HCC827 (EGFR del 19), and NCI-H838 [H838] (KRAS WT), human bronchial epithelial cell line HBE, and human embryonic kidney cell line HEK293T which is an excellent tool cell line for transfection and immunoprecipitation (IP) experiments were purchased from Procell (Wuhan, Hubei, China). The NSCLC cell lines and HBE cell line were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640, BasalMedia, Shanghai, China). The HEK293T cell line was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, BasalMedia). The mediums were supplemented with 10% fetal bovine serum (ExCell Bio, Shanghai, China) and 1% penicillin/streptomycin (Beyotime, Nantong, Jiangsu, China).
Nci h1299 h1299
Manufactured by Procell
Sourced in China
NCI-H1299 [H1299] is a human non-small cell lung cancer cell line. It is a commonly used in vitro model for cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Hcc827, by Procell (1 mentions)
Fetal bovine serum (fbs), by ExCell Bio (1 mentions)
Penicillin streptomycin, by Beyotime (1 mentions)
Rpmi 1640, by BasalMedia (1 mentions)
Hek293t, by Procell (1 mentions)
H1299, by Thermo Fisher Scientific (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using nci h1299 h1299
1
Isolation and Characterization of Human NSCLC Cell Lines
2
Cell Culture Protocols for Lung Cancer
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Human lung adenocarcinoma cells including NCI-H1299 (H1299) and A549 were purchased from Procell (Wuhan, China). Radioresistant H1299-RR cells were constructed in our laboratory as previously reported [23 (link)]. All cell lines were authenticated by short tandem repeat (STR) profiling and tested for mycoplasma contamination. H1299 and H1299-RR cells were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (#164,210–50, Procell, China) and 1% penicillin/streptomycin (P/S) (#BL505A, Biosharp, China). A549 cells were cultured in DMEM/F12 medium (Gibco, USA) supplemented with 10% FBS and 1% P/S. HEK293T cells were cultured in DMEM medium (Gibco, USA) supplemented with 10% FBS and 1% P/S. All cells were placed in the incubator at 37 ℃ in 5% CO2.
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