Cell apoptosis was assessed by measuring the membrane redistribution of phosphatidylserine using PE Annexin V Apoptosis Detection Kit l (BD Biosciences, Franklin Lakes, NJ, USA), according to the manufacturer’s instructions. Briefly, cells (4×106/well) were seeded into a T25 cell culture bottle. After transfection, the cells were cultured for 36 h. Subsequently, the cells were in the logarithmic phase, washed twice with cold PBS, resuspended in 1×Binding Buffer at a concentration of 1×106 cells/mL, and then 100 µL of the solution (1×105 cells) was transferred to a 2 mL culture tube. Finally, cells were stained with 5 µL of PE and 5 µL of 7-AAD, gently vortexed and incubated for 15 min at RT in the dark. Then, the cells were filtered with a filter screen and analysed using the BD FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). The cells in Q2 (late stage apoptosis) and Q4 (early stage apoptosis) area were all calculated as apoptotic cells.
Pe annexin 5 apoptosis detection kit l
Manufactured by BD
Sourced in United States
The PE Annexin V Apoptosis Detection Kit is a laboratory product that enables the detection and quantification of apoptosis, a programmed cell death process, in cell samples. The kit uses the Annexin V protein conjugated with the fluorescent dye phycoerythrin (PE) to detect the presence of phosphatidylserine, a molecule that becomes exposed on the cell surface during apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pe annexin 5 apoptosis detection kit l
1
Quantifying Cellular Apoptosis via Flow Cytometry
2
Apoptosis Analysis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flow cytometry analysis was done using PE Annexin V apoptosis detection kit l (BD Biosciences, Haryana, HR, USA), following manufacturer’s instructions. Briefly, cells were seeded into 12-well plates at 2.5 × 105 per well. After treatment, trypsinized cells were resuspended in 500 µL of Binding Buffer 1X and subsequently stained with annexin V-PE and 7AAD. Stained cells were evaluated with the BD Accuri C6 flow cytometer (BD Biosciences, Haryana, HR, USA) and analyzed using the FlowJo software (BD Biosciences, Haryana, HR, USA; version 10.8.1 for Windows).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!