Mouse il 6 elisa kit

Manufactured by Neobioscience

Sourced in China

The Mouse IL-6 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and measurement of mouse interleukin-6 (IL-6) in biological samples.

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Lab products found in correlation

Mouse kc il 8 cxcl1 elisa kit, by Neobioscience (2 mentions) Mouse il 1β uncoated elisa kit, by Thermo Fisher Scientific (2 mentions) Mouse tnf α elisa kit, by Neobioscience (1 mentions) Caspase 1 sc 514, by Santa Cruz Biotechnology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Ab19481, by Abcam (1 mentions) Ab22759, by Abcam (1 mentions) Ab48871, by Abcam (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Ab176347, by Abcam (1 mentions) Ab181389, by Abcam (1 mentions) Ab4207, by Abcam (1 mentions) Ab8245, by Abcam (1 mentions) Rat mouse insulin kit, by Merck Group (1 mentions)

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Mouse ELISA kits (16 citations)

7 protocols using mouse il 6 elisa kit

Quantifying Murine Skin Cytokines

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To detect murine IL-6, TNF-α, and IL-23 in skin lesions, a Mouse IL-6 ELISA kit (NeoBioscience cat. EMC004.96), a Mouse TNF-α ELISA kit (NeoBioscience cat. EMC102a.96), and a mouse IL-23 ELISA kit (BioLegend cat. 433704) were used.

Wang Z., Sun Y., Lou F., Bai J., Zhou H., Cai X., Sun L., Yin Q., Tang S., Wu Y., Fan L., Xu Z., Wang H., Hu X, & Wang H. (2022). Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis. Nature Communications, 13, 7815.

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Cytokine and Chemokine ELISA Assays

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ELISA was performed to test serum samples from patients and mice following the manufacturer’s protocol. The human-IL-6 kit (Cat.EHC007), mouse JE/MCP1/CCL2 kit (Cat.EMC113), mouse IL-6 ELISA kit (Cat.EMC004), and mouse KC/IL-8/CXCL1 ELISA kit (Cat.EMC104) were purchased from NeoBioscience Technology Company (ShenZhen, China). The rat/mouse insulin kit (Cat.EZRMI-13 K) was purchased from EMD Millipore Corporation. Mouse MMP12 ELISA kit (Cat.ARG81803, Arigo Biolaboratories) and human CXCL1/KC kit (Cat. EK-196, Multi Science Company). ELISA data were analyzed by Curve Expert 1.4 software.

Jiang L., Yang M., He S., Li Z., Li H., Niu T., Xie D., Mei Y., He X., Wei L., Huang P., Huang M., Zhang R., Wang L, & Li J. (2021). MMP12 knockout prevents weight and muscle loss in tumor-bearing mice. BMC Cancer, 21, 1297.

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Quantification of Cytokine Expression

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The supernatants of RAW 264.7 cells or blood from mice were collected to determine TNF-α or interleukin (IL)-6 expression by a commercial mouse TNF-α ELISA kit (4A BiotechCo. Ltd, Beijing, China) and a mouse IL-6 ELISA kit (Neobioscience, Shanghai, China), following the instructions in the manual.

Liu X., Xu Y., Li Y., Pan Y., Zhao S, & Hou Y. (2021). Ferumoxytol-β-glucan Inhibits Melanoma Growth via Interacting with Dectin-1 to Polarize Macrophages into M1 Phenotype. International Journal of Medical Sciences, 18(14), 3125-3139.

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Measuring Mouse Cytokine Levels

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Mouse IL-1β and IL-6 protein level was measured by Mouse IL-1β Uncoated ELISA Kit (Invitrogen) and mouse IL-6 ELISA Kit (EMC004, NeoBioscience) according to the manufacturers’ instructions.

Dou J.Y., Jiang Y.C., Hu Z.H., Yao K.C., Yuan M.H., Bao X.X., Zhou M.J., Liu Y., Li Z.X., Lian L.H., Nan J.X, & Wu Y.L. (2021). Betulin Targets Lipin1/2-Meidated P2X7 Receptor as a Therapeutic Approach to Attenuate Lipid Accumulation and Metaflammation. Biomolecules & Therapeutics, 30(3), 246-256.

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Quantifying IL-6 in ATDC5 cells

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IL-6 in the ATDC5 cell supernatant was quantitated by the mouse IL-6 ELISA kit (Neobioscience, Shenzhen, China) according to the manufacturer's instructions.

Dong X., Yang C., Luo Y., Dong W., Xu X., Wu Y, & Wang J. (2022). USP7 Attenuates Endoplasmic Reticulum Stress and NF-κB Signaling to Modulate Chondrocyte Proliferation, Apoptosis, and Inflammatory Response under Inflammation. Oxidative Medicine and Cellular Longevity, 2022, 1835900.

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Betulin Modulation of Inflammatory Pathways

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Betulin (BT) (BET201102212) was purchased from Skyherb Technologies (Hangzhou, China). Primary antibodies of SREBP1 (ab3259), lipin1 (ab181389), lipin2 (ab176347), P2X7r (ab48871), NLRP3 (ab4207), FANS (ab22759), PPARγ (ab19481) and GAPDH (ab8245) were purchased from Abcam (Cambridge, MA, USA). Primary antibodies of PPARα (sc-9000), PGC-1α (sc-518025), IL-6 (sc-28343), IL-18 (sc-133127), IL-1α (sc-393998) and caspase-1 (sc-514) were obtained from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA). Primary antibody of IL-1β (AF-401-NA) was purchased from R&D. Horseradish peroxidase (HRP)-conjugated goat anti-rabbit (ab97051) and rabbit anti-mouse (ab6728) were purchased from Abcam. Horseradish peroxidase (HRP)-conjugated rabbit anti-goat (HAF017) was purchased from R&D. The BCA Protein Assay Kit was obtained from Beyotime (Jiangsu, China). The Mouse IL-1β Uncoated ELISA Kit was purchased from Invitrogen (Carlsbad, CA, USA) and mouse IL-6 ELISA Kit was obtained from NeoBioscience (EMC004; Shenzhen, China). DMSO was purchased from Sigma Chemical Co (St. Louis, MO, USA). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle medium (DMEM) basic (1X) were purchased from Gibco (MA, USA). All other chemical reagents were analytical grade.

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Quantifying Cytokine Levels via ELISA

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The enzyme-linked immunosorbent assay (ELISA) was performed with kits with serum samples from patients and mice according to the manufacturer's protocol. The human-IL6 kit (EHC007), mouse JE/MCP1/CCL2 (EMC113) kit, mouse IL-6 ELISA kit (EMC004), and mouse KC/IL-8/CXCL1 ELISA kit (EMC104) were purchased from NeoBioscience Technology Company (ShenZhen, China). The rat/mouse insulin kit (EZRMI-13K) was purchased from EMD Millipore Corporation. A mouse MMP12 ELISA kit (ARG81803) was purchased from Arigobiolaboratories company. The human CXCL1/KC kit was purchased from Multi Science Company. The data of ELISA was analyzed by using Curve Expert1.4 software.

Jiang L., Yang M., He S., Li Z., Li H., Niu T., Xie D., Mei Y., He X., Wei L., Huang P., Huang M., Zhang R., Wang L., & Li J. (2021). MMP12 Knockout Prevent Weight and Muscle Loss Induced by Cancer Cachexia.

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