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Dna high sensitivity d5000 screentape

Manufactured by Agilent Technologies
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The DNA High Sensitivity D5000 ScreenTape is a laboratory instrument designed for the analysis of DNA samples. It provides automated, high-sensitivity electrophoretic separation and detection of DNA fragments ranging from 100 to 5,000 base pairs in length.

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Lab products found in correlation

Nextseq 500, by Illumina (3 mentions) Agilent tapestation, by Agilent Technologies (3 mentions) Quant seq 3 mrna seq library preparation kit, by Lexogen (3 mentions) Nextseq500 sequencing, by Illumina (1 mentions) Tapestation rna screentape, by Agilent Technologies (1 mentions) Rneasy plus mini kit, by Qiagen (1 mentions) Qubit 2.0 fluorometer, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

D5000 ScreenTape (45 citations) High Sensitivity DNA ScreenTape (13 citations) High Sensitivity D5000 ScreenTape (12 citations)

3 protocols using dna high sensitivity d5000 screentape

1

RNA Extraction and Sequencing Workflow

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RNA was extracted using the Qiagen RNeasy Plus mini kit (74134) after homogenization by centrifugation in QIAShredder (79656) microcentrifuge tubes. RNA quantity was determined using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific), and the quality was validated using the TapeStation RNA ScreenTape (Agilent). Two hundred nanograms of DNase I–treated total RNA was used to prepare the library for Illumina sequencing using a QuantSeq 3′ mRNA-Seq Library Preparation kit (Lexogen). Library quantity was determined using qPCR (KAPA Biosystem). Overall library size was determined using the Agilent TapeStation and the DNA High Sensitivity D5000 ScreenTape (Agilent). Equimolar amounts of each sample library were pooled, denatured, and sequenced using high-output, single-read, 75-bp-cycle Illumina NextSeq 500 sequencing kits. Next-generation sequencing was done on a NextSeq 500 (Illumina).
Wolpaw A.J., Grossmann L.D., Dessau J.L., Dong M.M., Aaron B.J., Brafford P.A., Volgina D., Pascual-Pasto G., Rodriguez-Garcia A., Uzun Y., Arsenian-Henriksson M., Powell DJ J.r., Bosse K.R., Kossenkov A., Tan K., Hogarty M.D., Maris J.M, & Dang C.V. (2022). Epigenetic state determines inflammatory sensing in neuroblastoma. Proceedings of the National Academy of Sciences of the United States of America, 119(6), e2102358119.
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2

Illumina Sequencing of 3'mRNA Library

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100 ng of DNAse treated total RNA was used to prepare library for Illumina sequencing using the Quant-Seq 3’mRNA-Seq Library Preparation Kit (Lexogen, Vienna, Austria). Library quantity was determined using qPCR (KAPA Biosystem, Wilmington, MA), and overall library size was resolved using the Agilent TapeStation and the DNA High Sensitivity D5000 ScreenTape (Agilent, Santa Clara, CA). Equimolar amount of libraries were pooled, denatured and High-Output, single read, 75 base pair Next Generation Sequencing was done on a NextSeq 500 (Illumina, San Diego, CA).
Giron L.B., Tanes C.E., Schleimann M.H., Engen P.A., Mattei L.M., Anzurez A., Damra M., Zhang H., Bittinger K., Bushman F., Kossenkov A., Denton P.W., Tateno H., Keshavarzian A., Landay A.L, & Abdel-Mohsen M. (2020). Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection. Mucosal Immunology, 13(5), 753-766.
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3

Illumina RNA-Seq Library Preparation

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100 ng of DNAse treated total RNA was used to prepare library for Illumina sequencing using the Quant-Seq 3’mRNA-Seq Library Preparation Kit (Lexogen, Vienna, Austria). Library quantity was determined using qPCR (KAPA Biosystem, Wilmington, MA), and overall library size was resolved using the Agilent TapeStation and the DNA High Sensitivity D5000 ScreenTape (Agilent, Santa Clara, CA). Equimolar amount of libraries were pooled, denatured and High-Output, single read, 75 base pair Next Generation Sequencing was done on a NextSeq 500 (Illumina, San Diego, CA).
Giron L.B., Tanes C.E., Schleimann M.H., Engen P.A., Mattei L.M., Anzurez A., Damra M., Zhang H., Bittinger K., Bushman F., Kossenkov A., Denton P.W., Tateno H., Keshavarzian A., Landay A.L, & Abdel-Mohsen M. (2020). Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection. Mucosal Immunology, 13(5), 753-766.
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