Alexa fluor 488 goat α mouse igg

Manufactured by Thermo Fisher Scientific

Alexa Fluor 488 goat α-mouse IgG is a fluorescently labeled secondary antibody. It is designed to detect and visualize mouse immunoglobulin G (IgG) in various immunoassays and other applications.

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Lab products found in correlation

S3007, by Merck Group (1 mentions) F3055, by Merck Group (1 mentions) Ab5095, by Abcam (1 mentions) Flavopiridol, by Merck Group (1 mentions) Ab5408, by Abcam (1 mentions) Ab817, by Abcam (1 mentions) Jbw301, by Merck Group (1 mentions) Ab104232, by Abcam (1 mentions) Ab15440, by Abcam (1 mentions) P1291, by Abcam (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Puromycin, by Merck Group (1 mentions) G3bp1, by Proteintech (1 mentions) Ythdf2, by Proteintech (1 mentions) β actin, by Merck Group (1 mentions) Dynein, by Santa Cruz Biotechnology (1 mentions) Ythdf3, by Santa Cruz Biotechnology (1 mentions) γ tubulin, by Santa Cruz Biotechnology (1 mentions) Gapdh, by AbFrontier (1 mentions) Lsm 710 confocal microscope, by Zeiss (1 mentions)

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Alexa Fluor 488 (6806 citations) Alexa 488 (1863 citations) Alexa Fluors (21 citations) Goat α-mouse (7 citations) Alexa-488 goat α-mouse (3 citations)

6 protocols using alexa fluor 488 goat α mouse igg

Chromatin Regulation Assays Using Antibodies

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The following chemicals were used in this study: flavopiridol (F3055, Sigma-Aldrich), radicicol (R-370, Alomone), sodium salicylate (S3007, Sigma-Aldrich), copper sulphate (102790, Merck).
The following primary antibodies were used in this study: α-mouse RNA polymerase II CTD repeat (Abcam, ab817), α-mouse RNA polymerase II CTD repeat phosphor S5 (Abcam, ab5408), α-rabbit RNA polymerase II CTD repeat phospho S2 (Abcam, ab5095), α-rat RNA polymerase II CTD repeat phosphor S2 (Active Motif, Cat#61083), α-rabbit HSF (gift from John Lis), α-rabbit Polyhomeotic (Paro lab stock), α-rabbit Pleiohomeotic (gift from Judith Kassis), α-rabbit Pleiohomeotic (gift from Jurg Muller), α-mouse FLAG (Sigma, F1804), α-rat HA (Roche, 11 867 423 001).
The following secondary antibodies were used in this study: α-mouse IgG HRP-linked whole antibody (GE Healthcare, NA931), α-rabbit IgG HRP-linked whole antibody (GE Healthcare, NA934V), α-rat IgG HRP-linked whole antibody (GE Healthcare, NA935V), Goat α-mouse IgG Alexa Fluor 488 (Thermo Fisher Scientific, A-11001), Goat α-rabbit IgG Alexa Fluor 568 (Thermo Fisher Scientific, A-11011).

Pereira A, & Paro R. (2017). Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus. Epigenetics & Chromatin, 10, 57.

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Antibody Characterization for Yeast Protein Analysis

Cited 1 time

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Polyclonal Smt3 (1:5,000), Slx5 (1:5,000), and Cdc48 (1:5,000) antibodies were raised in rabbits and have been described previously^{72 (link)–74 (link)}. Monoclonal antibodies directed against the HA epitope (1:1,000; 3F10) and monoclonal antibody against GFP (1:1,000; B-2) were purchased from Roche and Santa Cruz Biotechnology, respectively. Mouse monoclonal antibodies against Dpm1 (1:2,000; 5C5A7) and Pgk1 (1:5,000; 22C5D8) were obtained from Invitrogen. Mouse monoclonal antibodies against phospho-H2AX (Ser139) (1:1,000, JBW301) were obtained from Merck Millipore. Rabbit polyclonal antibodies against Nucleophosmin (1:100, ab15440) and Rad53 (1:1000, ab104232) or peroxidase antiperoxidase (1:1,000, p1291) were purchased from Abcam and Sigma-Aldrich, respectively. Secondary antibodies goat-α-mouse IgG Alexa fluor 488 (1:5,000; A11001) and donkey-α-rabbit IgG Alexa fluor 568 (1:5,000; A10042) were obtained from Thermo Fisher Scientific.

Capella M., Mandemaker I.K., Martín Caballero L., den Brave F., Pfander B., Ladurner A.G., Jentsch S, & Braun S. (2021). Nucleolar release of rDNA repeats for repair involves SUMO-mediated untethering by the Cdc48/p97 segregase. Nature Communications, 12, 4918.

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Antibody Validation for Protein Studies

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Antibodies against the following proteins (for immunostaining, western blotting, or IPs) were used in this study as described previously: SMG6^{74 (link)}, UPF1^{75 (link)}, and CTIF^{68 (link)}.
The purchased antibodies against the following proteins are listed in the format “protein name (catalog number, supplier)”: FLAG (DYKDDDDK; 14793, Cell Signaling Technology or A8592, Sigma-Aldrich), Myc (9E10; OP10L, Calbiochem or 2272, Cell Signaling Technology), FTO (ab124892, Abcam), GFP (sc-9996, Santa Cruz Biotechnology), DCTN1 (p150glued; 610474, BD Biosciences), eEF1A1 (CBP-KK1; EF1α; 05-235, Merck Millipore), YTHDF1 (17479-1-AP, Proteintech), YTHDF2 (24744-1-AP, Proteintech), YTHDF3 (sc-377119, Santa Cruz Biotechnology), METTL3 (15073-1-AP, Proteintech), METTL14 (HPA038002, Sigma-Aldrich), p-(S/T)Q ATM/ATR substrate (2851, Cell Signaling Technology), m⁶A (#202003, Synaptic Systems), m¹A (D345-3, MBL), puromycin (12D10; MABE343, Merck Millipore), β-actin (A5441, Sigma-Aldrich), GAPDH (LF-PA0212, AbFrontier), α-tubulin (sc-53030, Santa Cruz Biotechnology), γ-tubulin (sc-17788, Santa Cruz Biotechnology), dynein (sc-9115, Santa Cruz Biotechnology), G3BP1 (13057-2-AP, Proteintech), IMPβ (A301-803A-1, Bethyl Laboratories), Alexa Fluor 488 goat α-mouse IgG (A-11017, Invitrogen), and rhodamine-conjugated goat α-rabbit IgG (31670, Invitrogen).

Hwang H.J., Park T.L., Kim H.I., Park Y., Kim G., Song C., Cho W.K, & Kim Y.K. (2023). YTHDF2 facilitates aggresome formation via UPF1 in an m6A-independent manner. Nature Communications, 14, 6248.

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Immunohistochemical Analysis of Pancreatic Islets

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Pancreata were harvested and fixed in 4% PFA. Paraffin embedding, sectioning, and slide preparations were done in the SBP Histopathology Core Facility. Sections were stained with the following antibodies; α-glucagon (Abcam, K79bB10), α-PDIA1 (Proteintech, 11245–1-AP), α-proinsulin (HyTest Ltd., 2PR8, CCI-17), and DAPI (Fisher Scientific). Guinea pig polyclonal α-insulin antibody was produced in-house. For secondary antibodies, Alexa Fluor 488 goat α-rabbit IgG, Alexa Fluor 488 goat α-mouse IgG, Alexa Fluor 594 goat α-mouse IgG, and Alexa Fluor 594 goat α-guinea pig IgG antibodies were used (Invitrogen). Images were taken by Zeiss LSM 710 confocal microscope with a 40X objective lens. Scale bar, 20 µm.
For ß cell area measurement, pancreata were harvested, fixed in 4% PFA and embedded in paraffin. Three sections were prepared at 200 µm intervals for each pancreas and stained with guinea pig polyclonal insulin antibody and DAPI. Alexa Fluor 594 goat α-guinea pig IgG was used as a secondary antibody. Images were taken by an Aperio FL Scanner (Leica). Insulin stained ß cell area, islet area, and pancreas area were measured by Aperio Imagescope software.

Jang I., Pottekat A., Poothong J., Yong J., Lagunas-Acosta J., Charbono A., Chen Z., Scheuner D.L., Liu M., Itkin-Ansari P., Arvan P, & Kaufman R.J. (2019). PDIA1/P4HB is required for efficient proinsulin maturation and ß cell health in response to diet induced obesity. eLife, 8, e44528.

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Immunofluorescence Staining of Embryos

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Fixed embryos were rehydrated in 1 × PBS/0.5% Triton X-100 (PBSTx) and blocked with PBSTx/1% BSA/0.1% DMSO for 3 h. The embryos were then incubated with primary antibodies and secondary antibodies diluted in blocking solution for 2 h. Primary antibodies for GFP (IgG2a mouse monoclonal, Life technology), ZsYellow (anti-RCFP rabbit polyclonal antibody, Clontech), Nkx2.5 (Thermo Fisher, US) and Stat4 (Sigma, US) were incubated at a 1:50 dilution. Secondary antibodies (Alexa Fluor 488 goat α-mouse IgG, Alexa Fluor 546 goat-rabbit IgG (Invitrogen) were used at a 1:500 dilution.

Meng Z.Z., Liu W., Xia Y., Yin H.M., Zhang C.Y., Su D., Yan L.F., Gu A.H, & Zhou Y. (2017). The pro-inflammatory signalling regulator Stat4 promotes vasculogenesis of great vessels derived from endothelial precursors. Nature Communications, 8, 14640.

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TBX1 Transcription Factor Localization

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Jeg3 cells were grown in 10% FBS in DMEM, trypsinized and plated onto 6-well plates. Cells were transfected 24 hours later with 500 ng of Tbx1-pcDNA3.1, F148Y Tbx1-pCDNA3.1, H194Q Tbx1-pcDNA3.1, or G310S Tbx1-pcDNA3.1, and lipofectamine LTX (Invitrogen). Cells were fixed with 4% paraformaldehyde and 4% sucrose 48 hours later, for 15 minutes at room temperature. Cells were permeabilized with 0.3% Triton X-100 and blocked with 10% BSA/PBS for 30 minutes at 37°C. Cells were then incubated with rabbit polyclonal α mouse TBX1 1∶500 (Zymed) for two hours at 37°C and then with Alexa Fluor 488 goat α mouse IgG (Invitrogen) and DAPI stain (1∶500) for one hour at 37°C.

Castellanos R., Xie Q., Zheng D., Cvekl A, & Morrow B.E. (2014). Mammalian TBX1 Preferentially Binds and Regulates Downstream Targets Via a Tandem T-site Repeat. PLoS ONE, 9(5), e95151.

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