Ctan ctvol software

The fixed femurs were scanned using high-resolution μCT (Skyscan 1272; Kontich, Belgium) with a source voltage of 60 kV, current of 166 μA, and resolution of 14 μm. Bone morphometric parameters, including bone volume per total volume (BV/TV), bone mineral density (BMD), trabecular separation (Tb. Sp.), and trabecular number (Tb. N.) were analyzed using CTAn software (Bruker; Kontich, Belgium). Micro-CT 3D images of trabecular bones in the distal femurs were generated using CTAn/CTVol software (Bruker; Kontich, Belgium).
For histology, the fixed femurs were decalcified in 12% EDTA and embedded in paraffin, and the histological tissue sections were stained with hematoxylin and eosin and TRAP.

The fixed long bones were analyzed using a SkyScan 1272 high-resolution micro-CT system (Bruker, Kontich, Belgium). The scanning parameters were set at 60 kV/166 µA with a resolution of 8 µm for long bones. Three-dimensional images of the tibial trabecular bones (ROI: 0.21–1.94 mm from the growth plate) were obtained using CTAn/CTvol software (Bruker, Kontich, Belgium), and morphometric parameters including bone volume fraction (BV/TV), bone mineral density (BMD), trabecular separation (Tb. Sp.), and trabecular number (Tb. N.) were calculated using CTAn software (Bruker; Kontich, Belgium) as reported previously [31 (link),35 (link)]. For histological examination, the fixed long bones were decalcified in 10% EDTA, embedded in paraffin, and serial sections were cut to a thickness of 6 μm. The sections were stained with TRAP and used to quantify osteoclast number per bone parameter.