ChIP experiments were performed as previously described in33 (link) with minor modifications. Briefly, S.pombe cells were fixed with 1% formaldehyde for 15 min and then lysed in buffer containing 50 mM HEPES/KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na deoxycholate, 1 mM PMSF and protease-inhibitor cocktail. Chromatin was sheared with a Bioruptor (Diagenode). The following antibodies were used in this study: histone H3K9me2-specific mouse monoclonal antibody from Wako (clone no. MABI0307), histone H3-specific rabbit polyclonal antibody from Abcam (clone no. ab1791), histone H3K36me3-specific rabbit polyclonal antibody from Abcam (clone no. ab9050), and RNA Polymerase II mouse monoclonal antibody from Covance (clone no. 8WG16).
Histone h3k36me3 specific rabbit polyclonal antibody
Manufactured by Abcam
Histone H3K36me3-specific rabbit polyclonal antibody is a laboratory reagent that recognizes the trimethylated form of histone H3 at lysine 36. This modification is associated with active transcription and is involved in various cellular processes.
Automatically generated - may contain errors
2 protocols using histone h3k36me3 specific rabbit polyclonal antibody
1
ChIP Experiments in S. pombe
2
ChIP Experiments in S. pombe
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ChIP experiments were performed as previously described in33 (link) with minor modifications. Briefly, S.pombe cells were fixed with 1% formaldehyde for 15 min and then lysed in buffer containing 50 mM HEPES/KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na deoxycholate, 1 mM PMSF and protease-inhibitor cocktail. Chromatin was sheared with a Bioruptor (Diagenode). The following antibodies were used in this study: histone H3K9me2-specific mouse monoclonal antibody from Wako (clone no. MABI0307), histone H3-specific rabbit polyclonal antibody from Abcam (clone no. ab1791), histone H3K36me3-specific rabbit polyclonal antibody from Abcam (clone no. ab9050), and RNA Polymerase II mouse monoclonal antibody from Covance (clone no. 8WG16).
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